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The influence of UV exposure on 5-aminolevulinic acid-induced protoporphyrin IX production in skin
Department of Otolaryngology, Örebro Medical Center Hospital, Örebro, Sweden.
Biophysics Department, The Norwegian Radium Hospital, Oslo, Norway; 3Laser Research Center, Vilnius University, Vilnius, Lithuania.
Biophysics Department, The Norwegian Radium Hospital, Oslo, Norway.
Biophysics Department, The Norwegian Radium Hospital, Oslo, Norway.
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2001 (English)In: Photochemistry and Photobiology, ISSN 0031-8655, E-ISSN 1751-1097, Vol. 74, no 6, 825-828 p.Article in journal (Refereed) Published
Abstract [en]

The skin of nude mice was exposed to erythemogenic doses of UV radiation, which resulted in erythema with edema. An ointment containing 5-aminolevulinic acid (ALA) was topically applied on mouse and human skin. Differences in the kinetics of protoporphyrin accumulation were investigated in normal and UV-exposed skin. At 24 and 48 h after UV exposure, skin produced significantly less protoporphyrin IX (PpIX) than skin unexposed to UV. Human skin on body sites frequently exposed to solar radiation (the lower arm) also produced less PpIX than skin exposed more rarely to the sun (the upper arm). It is concluded that UV radiation introduces persisting changes in the skin, relevant to its capability of producing PpIX from ALA. The observed differences in ALA-induced PpIX fluorescence may be the result of altered penetration of ALA through the stratum corneum or altered metabolizing ability of normal and UV-exposed skin (or both).

Place, publisher, year, edition, pages
2001. Vol. 74, no 6, 825-828 p.
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Molecular Cellbiology; Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-38179DOI: 10.1562/0031-8655(2001)0740825TIOUEO2.0.CO2ISI: 000172863800012PubMedID: 11783939Scopus ID: 2-s2.0-0035790603OAI: oai:DiVA.org:oru-38179DiVA: diva2:758462
Note

The present work was supported by the Research Foundation of the Norwegian Radium Hospital and by the Örebro Medical Center Hospital. The authors are thankful to Even Angell-Petersen and Saulius Bagdonas for their help during the experiment.

Available from: 2014-10-27 Created: 2014-10-27 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Photodynamic therapy in the head and neck
Open this publication in new window or tab >>Photodynamic therapy in the head and neck
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Fotokemisk behandling av tumörer inom huvud- och halsområdet
Abstract [en]

Photodynamic therapy, PDT, is a method to diagnose and treat cancer. In PDT a sensitizer is administered to the patient and this sensitizer is accumulated in tumors. If the sensitizer-containing tumor is subjected to a laser of a specific wavelength the tumor is fluorescing allowing diagnostics. If other wavelengths are used a process involving reactive oxygen species and singlet oxygen is started and the tumor cells are killed. This process thus requires oxygen as well.

This thesis investigates how UV-induced damage of the skin and different physiological factors of the skin influences the uptake of 5- aminolevulinic acid, ALA, and its conversion to the active sensitizer protoporphyrin IX, PpIX. It shows that UV-induced damage affects both the uptake and production of PpIX. UV-induced damage lowers the PpIX produced after ALA application both if the damage is acute and in chronically UV-affected skin.

The PpIX production differs inter and intra individually. When looking how different physiological factors affect the PpIX production after topically applied ALA the thesis shows that an increase of temperature increases the production. No correlation between the formation of PpIX and the density of hair follicles was found and a weak correlation was seen comparing the epidermal and total dermal thickness and PpIX production

The thesis also shows how PDT is used in treating laryngeal malignancies. It shows that it is possible to cure laryngeal tumors (both squamous cell carcinomas and sarcomas) using PDT primarily, and that the cure rate as well as outcome of voice and patient safety is comparable to the conventional treatment modalities.

PDT can also be used as a function and organ sparing treatment for recurring laryngeal cancers, both squamous cell carcinomas and sarcomas.

Place, publisher, year, edition, pages
Örebro: Örebro university, 2014. 74 p.
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 110
Keyword
Photo Dynamic Therapy, Cancer, Skin, Larynx, 5-ALA, UVradiation, sarcoma, squamous cell carcinoma, porfimer sodium, temoporfin, voice
National Category
Surgery
Research subject
Surgery
Identifiers
urn:nbn:se:oru:diva-35953 (URN)978-91-7529-039-3 (ISBN)
Public defence
2014-09-19, Universitetssjukhuset, B-hus aulan, Södra Grev Rosengatan, Örebro, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2014-08-14 Created: 2014-08-14 Last updated: 2017-10-17Bibliographically approved

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von Beckerath, Mathias

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