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Influence of physiological parameters on the production of protoporphyrin IX in human skin by topical application of 5-aminolevulinic acid and its hexylester
The Head and Neck Oncology Center, Örebro University Hospital, Örebro, Sweden.
Department of Radiation Biology, The Norwegian Radium Hospital, Oslo, Norway.
The Head and Neck Oncology Center, Örebro University Hospital, Örebro, Sweden.
Department of Radiation Biology, The Norwegian Radium Hospital, Oslo, Norway.
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2006 (English)In: Journal of Medical Sciences, ISSN 1682-4474, E-ISSN 1812-5727, Vol. 6, no 4, 546-553 p.Article in journal (Refereed) Published
Abstract [en]

Formation of protoporphyrin IX (PpIX) after topical application of 5-aminolevulinic acid (ALA) and its hexylester derivative (ALA-Hex) was studied on healthy human skin. Temperature, density of hair follicles, epidermal and skin thickness were measured on the application sites. The skin temperature was found to be the strongest determinant for PpIX formation. The PpIX fluorescence increase was about 25% per degree Celsius. Formation of PpIX was found to be independent of the density of hair follicles. A weak correlation was found between the PpIX fluorescence and the thickness of epidermis and skin. Sun exposure seems to reduce the production of PpIX slightly.

Place, publisher, year, edition, pages
2006. Vol. 6, no 4, 546-553 p.
Keyword [en]
5-aminolevulinic acid esters, Epidermal thickness, Fluorescence, Hair follicles, Skin physiology, Skin thickness, Spectroscopy, Temperature, aminolevulinic acid, aminolevulinic acid hexyl ester, protoporphyrin, adult, article, controlled study, correlation analysis, density, epidermis, fluorescence analysis, hair follicle, human, human experiment, male, normal human, ointment, protein synthesis, skin temperature, skinfold thickness, sun exposure, volunteer
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Microbiology; Molecular Cellbiology
Identifiers
URN: urn:nbn:se:oru:diva-38183DOI: 10.3923/jms.2006.546.553ISI: -PubMedID: -Scopus ID: 2-s2.0-33750387149OAI: oai:DiVA.org:oru-38183DiVA: diva2:758512
Note

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Clinical research and future challenges (1997) Cancer, 79, pp. 2282-2308; Peng, Q., Warloe, T., Moan, J., Godal, A., Apricena, F., Giercksky, K.E., Nesland, J.M., Antitumor effect of 5-aminolevulinic acid-mediated photodynamic therapy can be enhanced by the use of a low dose of photofrin in human tumor xenografts (2001) Cancer Res., 61, pp. 5824-5832; Pottier, R.H., Chow, Y.F., LaPlante, J.P., Truscott, T.G., Kennedy, J.C., Beiner, L.A., Non-invasive technique for obtaining fluorescence excitation and emission spectra in vivo (1986) Photochem. Photobiol., 44, pp. 679-687; Sharfaei, S., Juzenas, P., Moan, J., Bissonnette, R., Weekly topical application of methyl aminolevulinate followed by light exposure delays the appearance of UV-induced skin tumours in mice (2002) Arch. Dermatol. Res., 294, pp. 237-242; Spikes, J.D., Photodynamic action: From Paramecium to Photochemotherapy (1997) Photochem. 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Available from: 2014-10-27 Created: 2014-10-27 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Photodynamic therapy in the head and neck
Open this publication in new window or tab >>Photodynamic therapy in the head and neck
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Fotokemisk behandling av tumörer inom huvud- och halsområdet
Abstract [en]

Photodynamic therapy, PDT, is a method to diagnose and treat cancer. In PDT a sensitizer is administered to the patient and this sensitizer is accumulated in tumors. If the sensitizer-containing tumor is subjected to a laser of a specific wavelength the tumor is fluorescing allowing diagnostics. If other wavelengths are used a process involving reactive oxygen species and singlet oxygen is started and the tumor cells are killed. This process thus requires oxygen as well.

This thesis investigates how UV-induced damage of the skin and different physiological factors of the skin influences the uptake of 5- aminolevulinic acid, ALA, and its conversion to the active sensitizer protoporphyrin IX, PpIX. It shows that UV-induced damage affects both the uptake and production of PpIX. UV-induced damage lowers the PpIX produced after ALA application both if the damage is acute and in chronically UV-affected skin.

The PpIX production differs inter and intra individually. When looking how different physiological factors affect the PpIX production after topically applied ALA the thesis shows that an increase of temperature increases the production. No correlation between the formation of PpIX and the density of hair follicles was found and a weak correlation was seen comparing the epidermal and total dermal thickness and PpIX production

The thesis also shows how PDT is used in treating laryngeal malignancies. It shows that it is possible to cure laryngeal tumors (both squamous cell carcinomas and sarcomas) using PDT primarily, and that the cure rate as well as outcome of voice and patient safety is comparable to the conventional treatment modalities.

PDT can also be used as a function and organ sparing treatment for recurring laryngeal cancers, both squamous cell carcinomas and sarcomas.

Place, publisher, year, edition, pages
Örebro: Örebro university, 2014. 74 p.
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 110
Keyword
Photo Dynamic Therapy, Cancer, Skin, Larynx, 5-ALA, UVradiation, sarcoma, squamous cell carcinoma, porfimer sodium, temoporfin, voice
National Category
Surgery
Research subject
Surgery
Identifiers
urn:nbn:se:oru:diva-35953 (URN)978-91-7529-039-3 (ISBN)
Public defence
2014-09-19, Universitetssjukhuset, B-hus aulan, Södra Grev Rosengatan, Örebro, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2014-08-14 Created: 2014-08-14 Last updated: 2017-10-17Bibliographically approved

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