To Örebro University

oru.seÖrebro University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Characterizing Bacillus amyloliquefaciens UCMB5113 on a Plant Model Arabidopsis thaliana
Örebro University, School of Science and Technology.
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Organic farming is gaining importance and acceptance worldwide due to its beneficial effects in agriculture and standing against losses caused by chemical fertilizers, pesticides and fungicides. Plant growth promoting bacteria (PGPB) plays an important role in organic farming by fixing atmospheric nitrogen, chelate iron, solubilizing phosphorous, producing and modulating phytohormones, providing antibiotics against pathogens. Understanding interaction mechanisms between PGPB and plant will be helpful in developing new formulations to form a strong symbiotic relationship between plant and bacteria. Bacillus amyloliquefaciens UCMB5113 is a red pigmented, rod shaped Gram positive bacteria which has been isolated from fields of the Ukraine. In the present study UCMB5113 and its interactions with the plant has been characterized. There was a significant promotion of plant root growth and protection against biotic stress with the application of 10 μl of 1x107/ml CFU UCMB5113 culture in Arabidopsis. The UCMB5113 can significantly withstand plant antimicrobial activity to stimulate plant root growth, but needs root hair defective RHD proteins to stimulate root hair elongation. UCMB5113 has significantly inhibited primary root elongation and developed number of lateral roots and root hairs in ethylene over expressed mutant, which suggests that it may be affecting ethylene signaling pathway in plants. UCMB5113 has a distinct red pigmentation which is a 38.5kDa water soluble protein with maximum absorbance at 422nm. These features are similar to the Orange Carotenoid Protein (OCP) of Synechocystis PCC 6803. This red pigmented protein has no significant effect on plant root growth promotion. Further biochemical and molecular studies are required to characterize and confirm the mechanisms of interaction.

Place, publisher, year, edition, pages
2014. , p. 15
Keywords [en]
Bacteria, Ethylene, Roots
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:oru:diva-38378OAI: oai:DiVA.org:oru-38378DiVA, id: diva2:761354
Subject / course
Biology
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2014-11-06 Created: 2014-11-06 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

fulltext(2098 kB)345 downloads
File information
File name FULLTEXT01.pdfFile size 2098 kBChecksum SHA-512
7558b731999424399fd1785fafdf2225cdac554c343ecb097a7bcddcf84bc1d1071809a60a8c3b0751da18a15ec64b3d4540dde1e784d6c2cb260bf4aa51b0b4
Type fulltextMimetype application/pdf

By organisation
School of Science and Technology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 345 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 418 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf