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Comparison of UVA- and UVA/riboflavin-induced growth inhibition of Acanthamoeba Castellanii
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Department of Ophthalmology, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Clinical Research Centre, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
Dept. Ophthalmology, Örebro University Hospital, Region Örebro County, Örebro, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Clinical Epidemiology and Biostatistic Unit, Örebro University Hospital, Örebro, Sweden.
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2013 (English)In: Graefe's Archives for Clinical and Experimental Ophthalmology, ISSN 0721-832X, E-ISSN 1435-702X, Vol. 251, no 2, p. 509-514Article in journal (Refereed) Published
Abstract [en]

Purpose To investigate whether ultraviolet light (UVA) at 365 nm can inhibit/eliminate Acanthamoeba growth and if riboflavin would potentiate such an association.

Method: Acanthamoeba castellanii in a fluid medium with a concentration of approximately 1.7 x 10(4) protozoa/ml were prepared with (0.01 %) and without riboflavin. Exposure of UVA (dose 5.475 J/cm(2)) took place twice, with each illumination period followed by culturing of 10 mu l in peptone yeast-extract glucose (PYG) medium for 7 days. Every suspension prepared had a non-exposed control solution. Determination of Acanthamoeba was conducted daily, by count in Burker chamber days 4 through 7 after exposure. Statistical analysis was done by repeated-measurement ANOVA and post-hoc analysis for unpaired samples.

Results: The exposure of ultraviolet light resulted in an inhibited growth of Acanthamoeba compared to the non-exposed solutions, with a statistically significant reduction over time (p = 0.0003). The addition of riboflavin did not amplify the effect, and there were no tendencies for an interaction effect between UVA and riboflavin. The antiprotozoal effect of the UVA wavelength, utilized in CXL, is solely mediated by ultraviolet light, and riboflavin does not seem to amplify the antimicrobial efficacy.

Place, publisher, year, edition, pages
New York, USA: Springer, 2013. Vol. 251, no 2, p. 509-514
Keywords [en]
Acanthamoeba, Acanthamoeba castellanii, UV, UVA, Riboflavin, Keratitis, Growth inhibition
National Category
Ophthalmology
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-38720DOI: 10.1007/s00417-012-2176-4ISI: 000314683200012PubMedID: 23079692Scopus ID: 2-s2.0-84877108959OAI: oai:DiVA.org:oru-38720DiVA, id: diva2:764156
Note

Funding agency:

Orebro University Hospital, Sweden: OLL-1578 , OLL-120691 

Available from: 2014-11-18 Created: 2014-11-18 Last updated: 2018-05-22Bibliographically approved

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Makdoumi, KarimBäckman, AndersCrafoord, Sven

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