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A combined DNA-microarray and mechanism-specific toxicity approach with zebrafish embryos to investigate the pollution of river sediments
Aquatic Ecology and Toxicology Group, COS–Center for Organismal Studies, University of Heidelberg, Heidelberg, Germany.
Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
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2012 (English)In: Reproductive Toxicology, ISSN 0890-6238, E-ISSN 1873-1708, Vol. 33, no 2, p. 245-253Article in journal (Refereed) Published
Abstract [en]

The zebrafish embryo has repeatedly proved to be a useful model for the analysis of effects by environmental toxicants. This proof-of-concept study was performed to investigate if an approach combining mechanism-specific bioassays with microarray techniques can obtain more in-depth insights into theecotoxicity of complex pollutant mixtures as present, e.g., in sediment extracts. For this end, altered gene expression was compared to data from established bioassays as well as to results from chemical analysis. Mechanism-specific biotests indicated a defined hazard potential of the sediment extracts, and microarray analysis revealed several classes of significantly regulated genes which could be related to the hazard potential. Results indicate that potential classes of contaminants can be assigned to sediment extracts by both classical biomarker genes and corresponding expression profile analyses of known substances. However, it is difficult to distinguish between specific responses and more universal detoxification of the organism.

Place, publisher, year, edition, pages
Oxford, United Kingdom: Elsevier, 2012. Vol. 33, no 2, p. 245-253
Keywords [en]
Microarray, Transcriptomics, Zebrafish, Sediment extract, Comet assay, EROD assay, Ames test, Neutral red assay
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:oru:diva-39949DOI: 10.1016/j.reprotox.2012.01.005ISI: 000302204700014PubMedID: 22326570Scopus ID: 2-s2.0-84862828537OAI: oai:DiVA.org:oru-39949DiVA, id: diva2:774004
Available from: 2014-12-20 Created: 2014-12-20 Last updated: 2018-05-13Bibliographically approved

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Keiter, Steffen

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