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Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection
Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany.
Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany.
Respiratory Diseases Clinic Heckeshorn, Department of Pneumology, HELIOS Klinikum Emil von Behring, Berlin, Germany.
Asklepios Professional Clinic München-Gauting, Centre for Pneumology and Thorax Surgery, Munich, Germany.
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2009 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 4, no 5, article id e5590Article in journal (Refereed) Published
Abstract [en]

Background: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans.

Methodology/Principal findings: We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection. Comparison between active pulmonary tuberculosis (TB) patients and healthy latently M. tuberculosis-infected donors (LTBI) revealed significantly higher T-cell responses against 7 of 35 tested M. tuberculosis latency-associated antigens in LTBI. Notably, T cells specific for Rv3407 were exclusively detected in LTBI but not in TB patients. The T-cell IFNgamma response against Rv3407 in individual donors was the most influential factor in discrimination analysis that classified TB patients and LTBI with 83% accuracy using cross-validation. Rv3407 peptide pool stimulations revealed distinct candidate epitopes in four LTBI.

Conclusions: Our findings further support the hypothesis that the latency-associated antigens can be exploited as biomarkers for LTBI.

Place, publisher, year, edition, pages
San Fransisco, USA: Public Library Science , 2009. Vol. 4, no 5, article id e5590
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Bioinformatics and Systems Biology
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URN: urn:nbn:se:oru:diva-40631DOI: 10.1371/journal.pone.0005590PubMedID: 19440342Scopus ID: 2-s2.0-65949089610OAI: oai:DiVA.org:oru-40631DiVA, id: diva2:778012
Available from: 2015-01-09 Created: 2015-01-09 Last updated: 2018-01-30Bibliographically approved

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