oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Two-color microarray experiments. Technology and sources of variance.
Institut für Medizinische Biometrie und Statistik, Universität zu Lübeck, Lübeck, Germany.ORCID iD: 0000-0002-7173-5579
Institut für Medizinische Biometrie und Statistik, Universität zu Lübeck, Lübeck, Germany .
2005 (English)In: Methods of Information in Medicine, ISSN 0026-1270, Vol. 44, no 3, p. 400-4Article in journal (Refereed) Published
Abstract [en]

Objectives: Microarray gene expression experiments have a complex technical background. Knowledge about certain technical details is inevitable to judge alternatives for both experimental design and analysis. Here, we introduce the necessary details for the so-called two-color microarray experiments and review major sources of technical variance.

Methods: We follow the sequence of experimental steps during a typical two-color microarray gene expression experiment, stressing decisive points in the choice of technique, experimental handling and biophysical basics. We point out where technical variation is to be expected.

Results: Tissue storage, RNA extraction techniques, as well as the microarray hybridization represent major components of technical variance to be considered. Depending on the possibilities for access to the biomedical material under investigation, choice of amplification and labeling techniques can also be decisive to avoid additional technical variance. The two-color microarray experimental approach seeks to avoid a group of probe-level technical biases making use of the advantages of an incomplete block-design.

Conclusions: It is worth to know the major sources of technical variance during the typical experimental sequence, both for choice of experimental design and techniques of molecular biology, as well as for the understanding of quality control and normalization approaches. Here, early investments pay at the level of reduced technical variance, allowing for enhanced detection levels for the effects under investigation.

Place, publisher, year, edition, pages
Stuttgart, Germany: Schattauer Gmbh, 2005. Vol. 44, no 3, p. 400-4
Keywords [en]
Agilent, chip, comparative hybridization, gene expression, microarray, technical variance
National Category
Bioinformatics and Systems Biology
Identifiers
URN: urn:nbn:se:oru:diva-40715ISI: 000230734600008PubMedID: 16113763Scopus ID: 2-s2.0-22944471092OAI: oai:DiVA.org:oru-40715DiVA, id: diva2:778523
Available from: 2015-01-10 Created: 2015-01-10 Last updated: 2018-01-30Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

PubMedScopusLänk till tidskrift

Authority records BETA

Repsilber, Dirk

Search in DiVA

By author/editor
Repsilber, Dirk
In the same journal
Methods of Information in Medicine
Bioinformatics and Systems Biology

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 68 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf