A greenhouse pot experiment was conducted to evaluate the feasibility of using Hieracium pilosella and soil microorganisms for phytostabilization of uranium-containing shale residues. Conductivity of leachates significantly decreased and pH increased when plants were grown on the substratum. H. pilosella has ability to change the hydrochemical parameters and to decrease the mobilization of uranium. Moreover, H. pilosella is able to accumulate significant amounts of uranium in the shoots.