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Microbe-host interactions in post-infectious irritable bowel syndrome
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.ORCID iD: 0000-0003-4713-1772
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro university , 2015. , p. 97
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 114
Keywords [en]
IBS, PI-IBS, Intestinal, Mucosa, Lymphocyte, Microbiota, Bacteria, Cytokine, Addaptive immune response, IL-13
National Category
Gastroenterology and Hepatology
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-41109ISBN: 978-91-7529-057-7 (print)OAI: oai:DiVA.org:oru-41109DiVA, id: diva2:779605
Public defence
2015-02-27, Prismahuset, Hörsal 2, Örebro universitet, Fakultetsgatan 1, Örebro, 09:00 (English)
Opponent
Supervisors
Available from: 2015-01-13 Created: 2015-01-13 Last updated: 2017-10-17Bibliographically approved
List of papers
1. Aberrant mucosal lymphocyte number and subsets in the colon of post-infectious irritable bowel syndrome patients
Open this publication in new window or tab >>Aberrant mucosal lymphocyte number and subsets in the colon of post-infectious irritable bowel syndrome patients
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2014 (English)In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 49, no 9, p. 1068-1075Article in journal (Refereed) Published
Abstract [en]

Background: Irritable bowel syndrome (IBS) is characterized by chronic abdominal symptoms such as pain, discomfort, and altered bowel habits. A subset of IBS patients, denoted as post-infectious IBS (PI-IBS) patients, develop symptoms after an enteric infection. Distinct abnormalities in the gut mucosa, including mucosal inflammation, have been proposed to contribute to or be the cause of PI-IBS. This study investigated lymphocyte subsets in PI-IBS patients compared to healthy controls.

Materials and methods: Ten PI-IBS patients and nine healthy controls participated. All PI-IBS patients met the Rome III diagnostic criteria for IBS and reported sustained symptoms at least 1 year after an episode of acute gastroenteritis. Intraepithelial lymphocytes and lamina propria lymphocytes (LPLs), isolated from mucosal tissue samples, were stained and analyzed for a comprehensive set of cell markers using flow cytometry.

Results: The number of LPLs in PI-IBS was significantly increased compared to those in healthy controls (p < 0.05). PI-IBS patients showed significantly increased proportions of CD45RO(+) CD4(+) activated/memory T cells (p < 0.05) and double-positive CD4(+) CD8(+) cells (p < 0.05), respectively, in the lamina propria. The number of CD19(+) LPLs was decreased in PI-IBS patients compared to healthy controls (p < 0.001).

Conclusion: This study presents new evidence that PI-IBS is associated with a sustained aberrant mucosal immune response and support future studies of anti-inflammatory or immune-modulating treatments in these patients.

Place, publisher, year, edition, pages
Informa Healthcare, 2014
Keywords
cell biology, gastrointestinal, infections, health economy, immunology
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-35383 (URN)10.3109/00365521.2014.926982 (DOI)000340829900006 ()24919810 (PubMedID)2-s2.0-84906318425 (Scopus ID)
Note

Funding Agency:

Medical Faculty, Örebro University

Available from: 2014-06-17 Created: 2014-06-17 Last updated: 2017-12-05Bibliographically approved
2. Altered faecal and mucosal microbial composition in post-infectious irritable bowel syndrome patients correlates with mucosal lymphocyte phenotypes and psychological distress
Open this publication in new window or tab >>Altered faecal and mucosal microbial composition in post-infectious irritable bowel syndrome patients correlates with mucosal lymphocyte phenotypes and psychological distress
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2015 (English)In: Alimentary Pharmacology and Therapeutics, ISSN 0269-2813, E-ISSN 1365-2036, Vol. 41, no 4, p. 342-351Article in journal (Refereed) Published
Abstract [en]

Background: A subset of irritable bowel syndrome (IBS) patients, denoted post-infectious IBS (PI-IBS), develop symptoms after an enteric infection. Bacterial dysbiosis and mucosal inflammation have been proposed to be involved in the pathophysiology of this entity.

Aim: To characterise the mucosal and faecal microbiota in PI-IBS, general IBS and healthy controls, and to investigate associations between the microbiota and the mucosal immune system.

Methods: Mucosal biopsies and faeces were collected from 13 PI-IBS patients, 19 general IBS patients and 16 healthy controls. Global bacterial composition was determined by generating 16S rRNA amplicons that were examined by phylogenetic microarray hybridisation, principal component and redundancy analysis. We correlated previously reported lymphocyte proportions with the microbiota.

Results: Faecal microbiota composition of PI-IBS patients differed significantly from both general IBS patients and healthy controls (P < 0.02). Both mucosal (P < 0.01) and faecal (P = 0.05) microbial diversity were reduced in PI-IBS compared to healthy controls. In the intraepithelial lymphocytes the previously published proportion of CD8+ CD45RA+ was negatively correlated with mucosal microbial diversity (P < 0.005). The previously published number of lamina propria lymphocytes was negatively correlated with mucosal microbial diversity (P < 0.05). Faecal microbial diversity was significantly negatively correlated with the Hospital Anxiety and Depression scale (P < 0.05).

Conclusions: We present data that distinguishes the intestinal microbiota of PI-IBS patients from that of both general IBS patients and HC. The microbial composition is significantly associated with the HADs score and alterations in lymphocyte subsets proportions.

Place, publisher, year, edition, pages
Hoboken, USA: Wiley-Blackwell, 2015
National Category
Cell and Molecular Biology Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-39955 (URN)10.1111/apt.13055 (DOI)000348731200002 ()25521822 (PubMedID)2-s2.0-84921438829 (Scopus ID)
Note

Funding Agencies:

Medical Faculty, Orebro University

Spinoza award of the Netherlands Organization for Scientific Research

Gravity grant (SIAM) of the Netherlands Organization for Scientific Research

Available from: 2014-12-22 Created: 2014-12-22 Last updated: 2018-01-11Bibliographically approved
3. Mucosal-associated microbiota differs less than fecal-associated microbiota between Irritable Bowel Syndrome patients and healthy subjects
Open this publication in new window or tab >>Mucosal-associated microbiota differs less than fecal-associated microbiota between Irritable Bowel Syndrome patients and healthy subjects
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(English)Manuscript (preprint) (Other academic)
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-43135 (URN)
Available from: 2015-03-02 Created: 2015-03-02 Last updated: 2017-10-17Bibliographically approved
4. Cytokine response after stimulation with key commensal bacteria differ in post-­infectious irritable bowel syndrome (PI-­IBS) patients compared to healthy subjects
Open this publication in new window or tab >>Cytokine response after stimulation with key commensal bacteria differ in post-­infectious irritable bowel syndrome (PI-­IBS) patients compared to healthy subjects
(English)Manuscript (preprint) (Other academic)
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-43133 (URN)
Available from: 2015-03-02 Created: 2015-03-02 Last updated: 2017-10-17Bibliographically approved

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Sundin, Johanna

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