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Leishmania donovani lipophosphoglycan inhibits phagosomal maturation via action on membrane rafts.
Linköping University, Linköping.
Linköping University, Linköping.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.ORCID iD: 0000-0002-4319-7208
INRS-Institut Armand-Frappier, Laval, QC, Canada .
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2009 (English)In: Microbes and infection, ISSN 1286-4579, E-ISSN 1769-714X, Vol. 11, no 2, p. 215-22Article in journal (Refereed) Published
Abstract [en]

Lipophosphoglycan (LPG), the major surface glycoconjugate on Leishmania donovani promastigotes, is crucial for the establishment of infection inside macrophages. LPG comprises a polymer of repeating Galbeta1,4Manalpha-PO(4) attached to a lysophosphatidylinositol membrane anchor. LPG is transferred from the parasite to the host macrophage membrane during phagocytosis and induces periphagosomal F-actin accumulation correlating with an inhibition of phagosomal maturation. The biophysical properties of LPG suggest that it may be intercalated into membrane rafts of the host-cell membrane. The aim of this study was to investigate if the effects of LPG on phagosomal maturation are mediated via action on membrane rafts. We show that LPG accumulates in rafts during phagocytosis of L. donovani and that disruption of membrane rafts abolished the effects of LPG on periphagosomal F-actin and phagosomal maturation, indicating that LPG requires intact membrane rafts to manipulate host-cell functions. We conclude that LPG associates with membrane rafts in the host cell and exert its actions on host-cell actin and phagosomal maturation through subversion of raft function.

Place, publisher, year, edition, pages
Paris, France: Elsevier, 2009. Vol. 11, no 2, p. 215-22
Keywords [en]
Leishmania, lipophosphoglycan, membrane rafts, phagosomal maturation, actin
National Category
Medical and Health Sciences Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:oru:diva-41299DOI: 10.1016/j.micinf.2008.11.007PubMedID: 19070677Scopus ID: 2-s2.0-61449144376OAI: oai:DiVA.org:oru-41299DiVA, id: diva2:780270
Available from: 2015-01-14 Created: 2015-01-14 Last updated: 2017-12-05Bibliographically approved

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Särndahl, Eva

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School of Health and Medical Sciences, Örebro University, Sweden
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Microbes and infection
Medical and Health SciencesMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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