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Differentiation of human dermal fibroblasts towards endothelial cells
Division of Plastic Surgery, Department of Surgery, Brigham and Women’s Hospital, Harvard Medical School, Boston, USA; Division of Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
Division of Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Plastic Surgery, County of Östergötland, Linköping, Sweden.
Division of Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Plastic Surgery, County of Östergötland, Linköping, Sweden.
Division of Surgery, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Plastic Surgery, County of Östergötland, Linköping, Sweden.
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2013 (English)In: Differentiation, ISSN 0301-4681, E-ISSN 1432-0436, Vol. 85, no 3, p. 67-77Article in journal (Refereed) Published
Abstract [en]

The ultimate goal of vascular tissue engineering is the production of functional grafts for clinical use. Difficulties acquiring autologous endothelial cells have motivated the search for alternative cell sources. Differentiation of dermal fibroblasts towards several mesenchymal lineages as well as endothelial cells has been proposed. The aim of the present study was to investigate the endothelial differentiation capacity of human dermal fibroblasts on a gene expression, protein expression and functional physiological level. Endothelial differentiation of fibroblasts was induced by culturing cells in 30% human serum, but not in fetal calf serum. Expression of proteins and genes relevant for endothelial function and differentiation was increased after induction. Furthermore, fibroblasts exposed to 30% human serum displayed increased uptake of low-density lipoprotein and formation of capillary-like networks. The results of this study may have an impact on cell sourcing for vascular tissue engineering, and the development of methods for vascularization of autologous tissue engineered constructs.

Place, publisher, year, edition, pages
Oxon, United Kingdom: Elsevier, 2013. Vol. 85, no 3, p. 67-77
Keywords [en]
Differentiation, Endothelial cell, Fibroblast, Tissue engineering, Human serum
National Category
Medical and Health Sciences Cell and Molecular Biology
Research subject
Biomedicine
Identifiers
URN: urn:nbn:se:oru:diva-41374DOI: 10.1016/j.diff.2013.01.005ISI: 000320766300001PubMedID: 23644553Scopus ID: 2-s2.0-84877357687OAI: oai:DiVA.org:oru-41374DiVA, id: diva2:780413
Available from: 2015-01-14 Created: 2015-01-14 Last updated: 2018-05-22Bibliographically approved

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