Binning metagenomic contigs by coverage and compositionShow others and affiliations
2014 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 11, no 11, p. 1144-6Article in journal (Refereed) Published
Abstract [en]
Shotgun sequencing enables the reconstruction of genomes from complex microbial communities, but because assembly does not reconstruct entire genomes, it is necessary to bin genome fragments. Here we present CONCOCT, a new algorithm that combines sequence composition and coverage across multiple samples, to automatically cluster contigs into genomes. We demonstrate high recall and precision on artificial as well as real human gut metagenome data sets.
Place, publisher, year, edition, pages
2014. Vol. 11, no 11, p. 1144-6
National Category
Bioinformatics (Computational Biology)
Identifiers
URN: urn:nbn:se:oru:diva-41485DOI: 10.1038/nmeth.3103ISI: 000344580600021PubMedID: 25218180Scopus ID: 2-s2.0-84908597294OAI: oai:DiVA.org:oru-41485DiVA, id: diva2:780487
Note
sponsors:
COST project Grant(s): ES1103
Swedish Research Councils VR Grant(s): 2011-5689
FORMAS Grant(s): 2009-1174
EC BONUS project BLUEPRINT
EPSRC Career Acceleration Fellowship Grant(s): EP/H003851/1
Unilever R&D Port Sunlight, Bebington, UK
Academy of Finland Grant(s): 256950
UK Medical Research Council Special Training Fellowship in Biomedical Informatics
UK National Institute for Health Research (NIHR) Centre for Surgical Reconstruction and Microbiology
NIHR Surgical Reconstruction and Microbiology Research Centre
Note: BILS (Bioinformatics Infrastructure for Life Sciences) is a distributed national research infrastructure with support from the Swedish Research Council. The aim of BILS is to provide bioinformatics support for life science researchers in Sweden.
2015-01-142015-01-142018-06-15Bibliographically approved