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MicroRNA expression profiling in endometrial adenocarcinoma
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro university , 2015. , 53 p.
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 118
Keyword [en]
Endometrial cancer, microRNA, BDII rat model, normalization, endogenous controls
National Category
Cancer and Oncology
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-41640ISBN: 9789175290638 (print)OAI: oai:DiVA.org:oru-41640DiVA: diva2:780763
Public defence
2015-03-27, Portalen, Högskolan i Skövde, Högskolevägen, Skövde, 09:00 (English)
Opponent
Supervisors
Available from: 2015-01-15 Created: 2015-01-15 Last updated: 2015-12-21Bibliographically approved
List of papers
1. miREC: a database of miRNAs involved in the development of endometrial cancer
Open this publication in new window or tab >>miREC: a database of miRNAs involved in the development of endometrial cancer
Show others...
(English)Article in journal (Refereed) Submitted
National Category
Cancer and Oncology
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-43411 (URN)
Available from: 2015-03-06 Created: 2015-03-06 Last updated: 2016-01-20Bibliographically approved
2. Validation of suitable endogenous control genes for quantitative PCR analysis of microRNA gene expression in a rat model of endometrial cancer
Open this publication in new window or tab >>Validation of suitable endogenous control genes for quantitative PCR analysis of microRNA gene expression in a rat model of endometrial cancer
2013 (English)In: Cancer Cell International, ISSN 1475-2867, E-ISSN 1475-2867, Vol. 13, 45Article in journal (Refereed) Published
Abstract [en]

Background: MicroRNAs are small RNA molecules that negatively regulate gene expression by translational inhibition or mRNA cleavage. The discovery that abnormal expression of particular miRNAs contributes to human disease, including cancer, has spurred growing interest in analysing expression profiles of these molecules. Quantitative polymerase chain reaction is frequently used for quantification of miRNA expression due to its sensitivity and specificity. To minimize experimental error in this system an appropriate endogenous control gene must be chosen. An ideal endogenous control gene should be expressed at a constant level across all samples and its expression stability should be unaffected by the experimental procedure.

Results: The expression and validation of candidate control genes (4.5S RNA(H) A, Y1, 4.5S RNA(H) B, snoRNA, U87 and U6) was examined in 21 rat cell lines to establish the most suitable endogenous control for miRNA analysis in a rat model of cancer. The stability of these genes was analysed using geNorm and NormFinder algorithms. U87 and snoRNA were identified as the most stable control genes, while Y1 was least stable.

Conclusion: This study identified the control gene that is most suitable for normalizing the miRNA expression data in rat. That reference gene will be useful when miRNAs expression are analyzed in order to find new miRNA markers for endometrial cancer in rat.

Keyword
Endogenous control genes, microRNA, Endometrial cancer
National Category
Cancer and Oncology
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-43404 (URN)10.1186/1475-2867-13-45 (DOI)000319504800001 ()23680393 (PubMedID)2-s2.0-84878248686 (Scopus ID)
Funder
Swedish Research Council, 2009/091
Note

Funding Agency: Nilsson-Ehle Foundation; Wilhelm and Martina Lundgren Foundation; Örebro University 

Available from: 2015-03-06 Created: 2015-03-06 Last updated: 2016-03-04Bibliographically approved
3. MicroRNA expression in human endometrial adenocarcinoma
Open this publication in new window or tab >>MicroRNA expression in human endometrial adenocarcinoma
2014 (English)In: Cancer Cell International, ISSN 1475-2867, E-ISSN 1475-2867, Vol. 14, no 1, 88Article in journal (Refereed) Published
Abstract [en]

Background: MicroRNAs are small non-coding RNAs that play crucial roles in the pathogenesis of different cancer types. The aim of this study was to identify miRNAs that are differentially expressed in endometrial adenocarcinoma compared to healthy endometrium. These miRNAs can potentially be used to develop a panel for classification and prognosis in order to better predict the progression of the disease and facilitate the choice of treatment strategy.

Methods Formalin fixed paraffin embedded endometrial tissue samples were collected from the Örebro university hospital. QPCR was used to quantify the expression levels of 742 miRNAs in 30 malignant and 20 normal endometrium samples. After normalization of the qPCR data, miRNAs differing significantly in expression between normal and cancer samples were identified, and hierarchical clustering analysis was used to identify groups of miRNAs with coordinated expression profiles.

Results: In comparisons between endometrial adenocarcinoma and normal endometrium samples 138 miRNAs were found to be significantly differentially expressed (p < 0.001) among which 112 miRNAs have not been previous reported for endometrial adenocarcinoma.

Conclusion: Our study shows that several miRNAs are differentially expressed in endometrial adenocarcinoma. These identified miRNA hold great potential as target for classification and prognosis of this disease. Further analysis of the differentially expressed miRNA and their target genes will help to derive new biomarkers that can be used for classification and prognosis of endometrial adenocarcinoma.

Place, publisher, year, edition, pages
London, United Kingdom: BioMed Central, 2014
Keyword
Endogenous control genes, microRNA, Endometrial cancer
National Category
Cancer and Oncology
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-43405 (URN)10.1186/s12935-014-0088-6 (DOI)000346199300001 ()25419182 (PubMedID)
Available from: 2015-03-06 Created: 2015-03-06 Last updated: 2017-03-01Bibliographically approved
4. Verification of miRNA expression in human endometrial adenocarcinoma
Open this publication in new window or tab >>Verification of miRNA expression in human endometrial adenocarcinoma
2014 (English)In: BMC Cancer, ISSN 1471-2407Article in journal (Other academic) Submitted
National Category
Cancer and Oncology
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-43413 (URN)
Available from: 2015-03-06 Created: 2015-03-06 Last updated: 2015-05-21Bibliographically approved

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