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Decreased density of muscarinic acetylcholine receptors in fibroblast from boys with attention deficit/hyperactivity disorder (ADHD): An in vitro study
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. (Experimentell neuropsykiatri)ORCID iD: 0000-0001-8102-1804
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. (Experimentell neuropsykiatri)
Skaraborg Hosp, Dept Pediat, Mariestad, Sweden.
Sahlgrens Acad, Gillberg Neuoropsychiat Ctr, Gothenburg, S.
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2012 (English)In: International Journal of Neuropsychopharmacology, ISSN 1461-1457, E-ISSN 1469-5111, Vol. 15, no s1, 224-224 p.Article in journal, Meeting abstract (Refereed) Published
Abstract [en]

Background: It is believed that the neurotransmitters, dopamine and norepinephrine are involved in the pathophysiology of the neurobehavioral disorder Attention Deficit/Hyperactivity Disorder (ADHD). Moreover, it is known that cholinergic activity can modulate dopaminergic activity in the brain. The aim of this study was to measure the density and affinity of muscarinic acetylcholine receptors (mAChRs) in children with ADHD, by using the fibroblast cell model.

Methods: Fibroblast cell homogenates from 11 boys with ADHD, fulfilling the DSM-IV diagnostic criteria and from 9 matching controls were used in the study. Radioligand binding assay was used to determine the maximal binding capacity (Bmax) and the equilibrium dissociation constant (KD) of mAChRs, using the mAChR antagonist 3H-QNB. Due to non-normally distribution of the calculated data, three outliers were identified by the MADE method (two in the ADHD group, both with a non-hereditary ADHD and one in the comparison group), and were therefore excluded from the statistical analyses.

Results: A significantly lower Bmax for the binding of the muscarinic antagonist 3H-QNB was observed in the fibroblasts from the children with ADHD (n=9) when compared to controls (n=8) (p=0.01), but the KD did not differ between the two groups (p=0.40).

Conclusions:The present results indicate a reduced density of mAChR in fibroblasts from children with a hereditary ADHD, which might be an indicator of the disorder. However, further studies are needed to confirm these observations.

Place, publisher, year, edition, pages
New York, USA: Cambridge University Press, 2012. Vol. 15, no s1, 224-224 p.
Keyword [en]
ADHD, Dopamine, Muscarinic receptor, Receptor binding assay, Fibroblast cells
National Category
Medical and Health Sciences Neurology Psychiatry
Research subject
Psychiatry; Biomedicine
Identifiers
URN: urn:nbn:se:oru:diva-42596ISI: 000209062500816OAI: oai:DiVA.org:oru-42596DiVA: diva2:787867
Conference
the 28th CINP World Congress of Neuropsychopharmacology, Stockholm, Sweden, 3–7 June 2012.
Available from: 2015-02-11 Created: 2015-02-11 Last updated: 2015-02-18Bibliographically approved

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Venizelos, NikolaosJohansson, Jessica
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