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Detection of polysaccharides and polysaccharide antibodies in pneumococcal pneumonia
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro university , 2015. , 93 p.
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 121
Keyword [en]
Streptococcus pneumoniae, pneumococcus, pneumonia, cellwall polysaccharide, capsular polysaccharide, antibody urinary antigen, nasopharyngeal aspirate, antibody, immunoglobulin, adult
National Category
Microbiology in the medical area
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-43010ISBN: 978-91-7529-073-7 (print)OAI: oai:DiVA.org:oru-43010DiVA: diva2:791312
Public defence
2015-05-08, Universitetssjukhuset, Bomanssonsalen, Södra Grev Rosengatan, Örebro, 09:00 (Swedish)
Opponent
Supervisors
Note

Funding: Research Committee of Region Orebro County Council

Available from: 2015-02-27 Created: 2015-02-27 Last updated: 2015-12-21Bibliographically approved
List of papers
1. The Uni-Gold™ Streptococcus pneumoniae urinary antigen test: an inter-assay comparison with the BinaxNOW® Streptococcus pneumoniae test on consecutive urine samples and evaluation on patients with bacteremia
Open this publication in new window or tab >>The Uni-Gold™ Streptococcus pneumoniae urinary antigen test: an inter-assay comparison with the BinaxNOW® Streptococcus pneumoniae test on consecutive urine samples and evaluation on patients with bacteremia
Show others...
(English)Manuscript (preprint) (Other academic)
National Category
Infectious Medicine
Identifiers
urn:nbn:se:oru:diva-44401 (URN)
Available from: 2015-04-22 Created: 2015-04-22 Last updated: 2015-04-22Bibliographically approved
2. The Binax NOW Streptococcus pneumoniae test applied on nasopharyngeal aspirates to support pneumococcal aetiology in community-acquired pneumonia.
Open this publication in new window or tab >>The Binax NOW Streptococcus pneumoniae test applied on nasopharyngeal aspirates to support pneumococcal aetiology in community-acquired pneumonia.
2013 (English)In: Scandinavian Journal of Infectious Diseases, ISSN 0036-5548, E-ISSN 1651-1980, Vol. 45, no 6, 425-31 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The use of nasopharyngeal secretions to enhance diagnostic yields of pneumococcal aetiology in community-acquired pneumonia (CAP) is of interest. We evaluated the Binax NOW Streptococcus pneumoniae immunochromatographic test (ICT) on nasopharyngeal aspirates (NPA) in order to support pneumococcal aetiology in CAP.

METHODS: The NPA ICT was applied on 180 adult CAP patients and 64 healthy controls. The rate of pneumococcal detection in the nasopharynx was compared to rates for lytA polymerase chain reaction (PCR) and culture on NPA.

RESULTS: According to blood and sputum culture and urine ICT, the test sensitivity in 59 patients with a pneumococcal aetiology was 81%. The specificity was suboptimal, with 72% negative tests among CAP patients without a pneumococcal aetiology. However, the test was positive in only 11% of patients with atypical pneumonia and in 4.7% of healthy controls. The positivity rate was higher for NPA ICT compared to culture on NPA in all CAP patients, and to both PCR and culture on NPA in non-pneumococcal non-atypical CAP patients. In 113 (63%) patients with β-lactam monotherapy, cure without treatment alteration was noted more often in cases with positive compared to negative NPA ICT at admission (91% vs 69%; p < 0.01).

CONCLUSIONS: The high sensitivity and the low positivity rates in patients with atypical pneumonia and healthy controls, in combination with the correlation between positive test results and clinical cure with β-lactam therapy, may support a pneumococcal aetiology in CAP in populations with low pneumococcal carriage rates.

National Category
Infectious Medicine
Identifiers
urn:nbn:se:oru:diva-44372 (URN)10.3109/00365548.2012.760843 (DOI)000318940400002 ()23330980 (PubMedID)2-s2.0-84877917212 (Scopus ID)
Note

Funding Agency: Research Committee of Orebro County Council

Available from: 2015-04-22 Created: 2015-04-20 Last updated: 2017-03-16Bibliographically approved
3. Clinical and microbiological factors associated with high pneumococcal colonization density in pneumococcal pneumonia
Open this publication in new window or tab >>Clinical and microbiological factors associated with high pneumococcal colonization density in pneumococcal pneumonia
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(English)Manuscript (preprint) (Other academic)
National Category
Infectious Medicine
Identifiers
urn:nbn:se:oru:diva-44402 (URN)
Available from: 2015-04-22 Created: 2015-04-22 Last updated: 2015-04-22Bibliographically approved
4. Association between serotype-specific antibody response and serotype characteristics in patients with pneumococcal pneumonia, with special reference to degree of encapsulation and invasive potential
Open this publication in new window or tab >>Association between serotype-specific antibody response and serotype characteristics in patients with pneumococcal pneumonia, with special reference to degree of encapsulation and invasive potential
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2014 (English)In: Clinical and Laboratory Immunology, ISSN 1556-6811, E-ISSN 1556-679X, Vol. 21, no 11, 1541-1549 p.Article in journal (Refereed) Published
Abstract [en]

We studied the immunoglobulin (Ig) response to causative serotype-specific capsular polysaccharides in adult pneumococcal pneumonia patients. The serotypes were grouped according to their degree of encapsulation and invasive potential. Seventy patients with pneumococcal pneumonia, 20 of whom were bacteremic, were prospectively studied. All pneumococcal isolates from the patients were serotyped, and the Ig titers to the homologous serotype were determined in acute- and convalescent-phase sera using a serotype-specific enzyme-linked immunosorbent assay. The Ig titers were lower in bacteremic cases than in nonbacteremic cases (P < 0.042). The Ig titer ratio (convalescent/acute titer) was ≥2 in 33 patients, 1 to 1.99 in 20 patients, and <1 in 17 patients. Patients ≥65 years old had a lower median Ig titer ratio than did younger patients (P < 0.031). The patients with serotypes with a thin capsule (1, 4, 7F, 9N, 9V, and 14) and medium/high invasive potential (1, 4, 7F, 9N, 9V, 14, and 18C) had higher Ig titer ratios than did patients with serotypes with a thick capsule (3, 6B, 11A, 18C, 19A, 19F, and 23F) and low invasive potential (3, 6B, 19A, 19F, and 23F) (P < 0.05 for both comparisons after adjustment for age). Ig titer ratios of <1 were predominantly noted in patients with serotypes with a thick capsule. In 8 patients with pneumococcal DNA detected in plasma, the three patients with the highest DNA load had the lowest Ig titer ratios. In conclusion, a high antibody response was associated with serotypes with a thin capsule and medium/high invasive potential, although a low antibody response was associated with serotypes with a thick capsule and a high pneumococcal plasma load.

Place, publisher, year, edition, pages
American Society for Microbiology, 2014
National Category
Infectious Medicine Immunology in the medical area
Identifiers
urn:nbn:se:oru:diva-44373 (URN)10.1128/CVI.00259-14 (DOI)000344651200011 ()25230937 (PubMedID)2-s2.0-84914697787 (Scopus ID)
Note

Funding Agency: Research Committee of Orebro County Council OLL-137011

Available from: 2015-04-22 Created: 2015-04-20 Last updated: 2017-03-24Bibliographically approved

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