oru.sePublikationer
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Elevated plasma levels of lipopolysaccharide and high mobility group box-1 protein are associated with high viral load in HIV-1 infection: reduction by 2-year antiretroviral therapy
Department of Clinical Virology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden; Department of Infectious Diseases, Oslo University Hospital, Ullevål, Oslo, Norway .
Department of Clinical Virology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden; Department of Infectious Diseases, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden .
Department of Clinical Virology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden.
Department of Clinical Virology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Sweden.
Show others and affiliations
2010 (English)In: AIDS (London), ISSN 0269-9370, E-ISSN 1473-5571, Vol. 24, no 11, 1733-1737 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: To investigate plasma levels of high mobility group box-1 protein (HMGB1), a marker of tissue necrosis and immune activation, as well as lipopolysaccharide (LPS), a marker of bacterial translocation, in HIV-1-infected patients.

DESIGN: We studied 32 HIV-1-positive patients who had responded to antiretroviral therapy with undetectable viremia after 2 years, 10 nonresponders and 19 healthy controls.

METHODS: HMGB1 was analyzed by ELISA, and LPS by Lamilus colometric assay. Nonparametric statistics were applied.

RESULTS: In naive HIV-1 patients, HMGB1 and LPS were elevated as compared with controls (P < 0.001). LPS levels were higher in African and Oriental patients compared with whites (P = 0.007). Notably, viral load was two-fold higher in patients with LPS, and HMGB1 was above median as compared with other patients (P = 0.005). This association was largely driven by African patients, who had a five-fold increased viral load in the presence of elevated LPS and HMGB1. After 2 years of effective antiretroviral therapy, LPS was reduced to the same median level as in the control group (P < 0.001), and HMGB1 was also reduced (P = 0.001), whereas no reductions were seen in nonresponders.

CONCLUSION: The new findings are the association of elevated plasma levels of LPS and HMGB1 with high viral load, as well as the normalized levels of LPS, and the reduction of HMGB1 after 2 years of effective antiretroviral therapy. As LPS and HMGB1 tend to form immunologically active complexes in vitro, we propose that such complexes may be involved in the immune activation and pathogenesis of HIV-1 infection.

Place, publisher, year, edition, pages
2010. Vol. 24, no 11, 1733-1737 p.
Keyword [en]
antiretroviral therapy; high mobility group box-1 protein; HIV-1; immune activation; lipopolysaccharide; microbial translocation
National Category
Infectious Medicine Immunology in the medical area
Research subject
Infectious Diseases; Immunology
Identifiers
URN: urn:nbn:se:oru:diva-41994DOI: 10.1097/QAD.0b013e32833b254dISI: 000279396600014PubMedID: 20502315Scopus ID: 2-s2.0-77954349457OAI: oai:DiVA.org:oru-41994DiVA: diva2:792891
Note

Funding Agencies:

Swedish Medical Research Council

Karolinska Institutet

Swedish International Developing Agency

Available from: 2015-03-05 Created: 2015-01-16 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Abdurahman, Samir
In the same journal
AIDS (London)
Infectious MedicineImmunology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 167 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf