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A combined culture and PCR method for detection of pathogenic Yersinia enterocolitica in food
National Food Administration, Uppsala, Sweden .
National Food Administration, Uppsala, Sweden .
Microbiology Department, Uppsala University, Uppsala, Sweden .
Department of Food Hygiene, Swed. Univ. of Agricultural Sciences, Uppsala, Sweden.ORCID iD: 0000-0003-0154-9452
2000 (English)In: International Journal of Food Microbiology, ISSN 0168-1605, E-ISSN 1879-3460, Vol. 57, no 1-2, 63-73 p.Article in journal (Refereed) Published
Abstract [en]

A combined method based on traditional culturing, buoyant density centrifugation, (BDC), and polymerase chain reaction (PCR) techniques for detection and identification of pathogenic Y. enterocolitica in food was developed and evaluated. An internal control, which was added in each PCR-tube and co-amplified by the same primer pair as the pathogen, monitored false-negative PCR results. The sample preparation step, BDC, was used to remove PCR inhibiting food substances and to concentrate the Y. enterocolitica cells. Single PCR with a chromosomal gene (ail) as target was chosen for screening the samples. The method was tested on naturally and artificially contaminated food samples. In three different food samples, processed meat (brawn), unprocessed beef and minced pork, inoculated with 10 cfu pathogenic Y. enterocolitica per gram, Y. enterocolitica was detected and cultural bacteria indicated within 18 h of enrichment.

Place, publisher, year, edition, pages
2000. Vol. 57, no 1-2, 63-73 p.
Keyword [en]
Buoyant density centrifugation; Food; Pathogenic Y. enterocolitica; PCR
National Category
Other Social Sciences not elsewhere specified Microbiology
Research subject
Culinary Arts and Meal Science; Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-43712DOI: 10.1016/S0168-1605(00)00232-4ISI: 000087552200007Scopus ID: 2-s2.0-0034631288OAI: oai:DiVA.org:oru-43712DiVA: diva2:795935
Available from: 2015-03-17 Created: 2015-03-17 Last updated: 2017-10-17Bibliographically approved

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Danielsson-Tham, Marie-Louise
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CiteExportLink to record
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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
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  • Other style
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Language
  • de-DE
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  • en-US
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  • nn-NO
  • nn-NB
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Output format
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