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Effects of ΔNp73β on cisplatin treatment in colon cancer cells
University of Skövde.
Linköping University.
Linköping University.
Linköping University.
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2012 (English)In: Molecular Carcinogenesis, ISSN 0899-1987, E-ISSN 1098-2744, Vol. 51, no 8, 628-35 p.Article in journal (Refereed) Published
Abstract [en]

p73 can activate transcription of p53-responsive genes, thereby inhibiting cell growth. An alternative promoter in the TP73 gene gives rise to an N-terminally truncated isoform of p73, ΔNp73, which lacks the transactivation domain of the full length TAp73 protein. TAp73 is considered pro-apoptotic, and ΔNp73 anti-apoptotic. In this study, we overexpressed ΔNp73β in p53 wild type and p53 mutant colon cancer cell lines and further exposed the cells to cancer therapeutic drug cisplatin. The results showed that cisplatin decreased the protein expression levels of ΔNp73β in a dose-dependent manner, and both TAp73 and p53 were upregulated after cisplatin treatment. Further, clonogenic potential and cell viability were decreased, and apoptotic cells increased, in p53 mutant and in p53 wild type cells. Cellular viability was significantly higher in ΔNp73β-cells than mock-transfected cells. However, ΔNp73β overexpression did not affect the cellular susceptibility to cisplatin. In conclusion, the overexpression of ΔNp73β increases viability in p53 wild type and p53 mutant colon cancer cells, and cisplatin induces the degradation of ΔNp73β in a dose-dependent manner.

Place, publisher, year, edition, pages
2012. Vol. 51, no 8, 628-35 p.
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:oru:diva-44362DOI: 10.1002/mc.20835ISI: 000305962400004PubMedID: 21837762Scopus ID: 2-s2.0-84863477921OAI: oai:DiVA.org:oru-44362DiVA: diva2:806490
Funder
Swedish Cancer SocietySwedish Research Council
Note

Funding Agency: Health Research Council in the South-East of Sweden 

Available from: 2015-04-20 Created: 2015-04-20 Last updated: 2015-04-20Bibliographically approved
In thesis
1. Molecular studies of radiotherapy and chemotherapy in colorectal cancer
Open this publication in new window or tab >>Molecular studies of radiotherapy and chemotherapy in colorectal cancer
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro university, 2015. 73 p.
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 122
Keyword
Colorectal cancer, Radiotherapy, Chemotherapy, p53, p73, PINCH, miRNAs
National Category
Medical Biotechnology
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-43635 (URN)978-91-7529-070-6 (ISBN)
Public defence
2015-05-29, Prismahuset, Hörsal 2, Örebro universitet, Fakultetsgatan 1, Örebro, 13:00 (Swedish)
Opponent
Supervisors
Funder
Swedish Cancer SocietySwedish Research Council
Note

Funding Agency:

Health Research Council in the South-East of Sweden

Available from: 2015-03-16 Created: 2015-03-16 Last updated: 2015-12-21Bibliographically approved

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