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Identification and characterization of pathogenic Yersinia enterocolitica isolates by PCR and pulse-field get electrophoresis
Research and Development Department, National Food Administration, Uppsala; .
Department of Food Hygiene, Swedish University of Agricultural Sciences, Uppsala.ORCID iD: 0000-0003-0154-9452
2005 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 71, no 7, 3674-3681 p.Article in journal (Refereed) Published
Abstract [en]

Approximately 550 to 600 yersiniosis patients are reported annually in Sweden. Although pigs are thought to be the main reservoir of food-borne pathogenic Yersinia enterocolitica, the role of pork meat as a vehicle for transmission to humans is still unclear. Pork meat collected from refrigerators and local shops frequented by yersiniosis patients (n = 48) were examined for the presence of pathogenic Yersinia spp. A combined culture and PCR method was used for detection, and a multiplex PCR was developed and evaluated as a tool for efficient identification of pathogenic food and patient isolates. The results obtained with the multiplex PCR were compared to phenotypic test results and confirmed by pulsed-field gel electrophoresis (PFGE). In all, 118 pork products (91 raw and 27 ready-to-eat) were collected. Pathogenic Yersinia spp. were detected by PCR in 10% (9 of 91) of the raw pork samples (loin of pork, fillet of pork, pork chop, ham, and minced meat) but in none of the ready-to-eat products. Isolates of Y. enterocolitica bioserotype 4/O:3 were recovered from six of the PCR-positive raw pork samples; all harbored the virulence plasmid. All isolates were recovered from food collected in shops and, thus, none were from the patients' home. When subjected to PFGE, the six isolates displayed four different NotI profiles. The same four NotI profiles were also present among isolates recovered from the yersiniosis patients. The application of a multiplex PCR was shown to be an efficient tool for identification of pathogenic Y. enterocolitica isolates in naturally contaminated raw pork.

Place, publisher, year, edition, pages
Washington, USA: American Society for Microbiology , 2005. Vol. 71, no 7, 3674-3681 p.
National Category
Medical and Health Sciences Microbiology
Research subject
Culinary Arts and Meal Science
Identifiers
URN: urn:nbn:se:oru:diva-44379DOI: 10.1128/AEM.71.7.3674-3681.2005ISI: 000230445700037PubMedID: 16000776Scopus ID: 2-s2.0-22144448253OAI: oai:DiVA.org:oru-44379DiVA: diva2:806613
Available from: 2015-04-21 Created: 2015-04-21 Last updated: 2017-10-17Bibliographically approved

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