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Division of Human Listeria monocytogenes Pulsed-Field Gel Electrophoresis (PFGE) Types Belonging to Lineage I (Serovar 4b, 1/2b, and 3b) into PFGE Groups
Örebro University, School of Hospitality, Culinary Arts & Meal Science.
Örebro University, School of Hospitality, Culinary Arts & Meal Science.ORCID iD: 0000-0003-0154-9452
Creighton Univ, Med Ctr, Sch Med, Dept Med Microbiol & Immunol, Omaha, CA USA.
Örebro University, School of Hospitality, Culinary Arts & Meal Science.ORCID iD: 0000-0001-6903-5322
2015 (English)In: Foodborne pathogens and disease, ISSN 1535-3141, E-ISSN 1556-7125, Vol. 12, no 5, 447-453 p.Article in journal (Refereed) Published
Abstract [en]

The 63 pulsed-field gel electrophoresis (PFGE) types identified among 427 clinical isolates of Listeria monocytogenes that were characterized in a previous study by serotyping and PFGE (AscI) could be further divided into 17 PFGE groups. While the 63 PFGE types, all part of lineage I, were established based on the number and distribution of all bands in each DNA profile, the 17 PFGE groups were based on the configuration of small bands with sizes L. monocytogenes serovar 4b isolates (n=334) were divided into 8 PFGE groups; the 32 PFGE types of serovar 1/2b isolates (n=90) and the serovar 3b isolates (n=3, 1 PFGE type) were divided into 9 PFGE groups. An association was observed between PFGE groups and serovars. L. monocytogenes isolates belonging to PFGE groups I, J, Q, R, X, Z, o-4, and o-5 all shared serovar 4b, whereas isolates from PFGE groups D, G, O, P, T, U, o-1, o-2, and o-3 shared serovar 1/2b. Small fragments L. monocytogenes isolates. From the results of the present study, a procedure for accelerating the identification of PFGE types when analyzing new PFGE profiles can be suggested. Therefore, we propose a stepwise procedure to PFGE profiling by first identifying the PFGE group using the smaller band patterns <145.5 kb, and then determining PFGE types based on the band patterns >145.5 kb.

Place, publisher, year, edition, pages
Mary Ann Liebert, 2015. Vol. 12, no 5, 447-453 p.
National Category
Other Social Sciences not elsewhere specified
Research subject
Culinary Arts and Meal Science
Identifiers
URN: urn:nbn:se:oru:diva-44674DOI: 10.1089/fpd.2014.1880ISI: 000353777800010PubMedID: 25803595OAI: oai:DiVA.org:oru-44674DiVA: diva2:813273
Note

Funding Agency:

Stiftelsen Grythytte Akademi Stipendiefond

Available from: 2015-05-22 Created: 2015-05-22 Last updated: 2017-10-18Bibliographically approved

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Lopez-Valladares, GloriaDanielsson-Tham, Marie-LouiseTham, Wilhelm
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