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Sequence types of Staphylococcus epidermidis associated with prosthetic joint infections are not present in the laminar airflow during prosthetic joint surgery
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Centre for Clinical Research, Uppsala University, Uppsala; County Hospital, Västerås, Sweden.ORCID iD: 0000-0003-2867-1044
Örebro University Hospital. Department of Laboratory Medicine, Clinical Microbiology.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
Örebro University, School of Medicine, Örebro University, Sweden.ORCID iD: 0000-0001-5939-2932
2015 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 123, no 7, 589-595 p.Article in journal (Refereed) Published
Abstract [en]

Molecular characterization of Staphylococcus epidermidis isolates from prosthetic joint infections (PJIs) has demonstrated a predominance of healthcare-associated multi-drug resistant sequence types (ST2 and ST215). How, and when, patients acquire these nosocomial STs is not known. The aim was to investigate if sequence types of S. epidermidis associated with PJIs are found in the air during prosthetic joint surgery. Air sampling was undertaken during 17 hip/knee arthroplasties performed in operating theaters equipped with mobile laminar airflow units in a 500-bed hospital in central Sweden. Species identification was performed using MALDI-TOF MS and 16S rRNA gene analysis. Isolates identified as S. epidermidis were further characterized by MLST and antibiotic susceptibility testing. Seven hundred and thirty-five isolates were available for species identification. Micrococcus spp. (n = 303) and coagulase-negative staphylococci (n = 217) constituted the majority of the isolates. Thirty-two isolates of S. epidermidis were found. S. epidermidis isolates demonstrated a high level of allelic diversity with 18 different sequence types, but neither ST2 nor ST215 was found. Commensals with low pathogenic potential dominated among the airborne microorganisms in the operating field during prosthetic joint surgery. Nosocomial sequence types of S. epidermidis associated with PJIs were not found, and other routes of inoculation are therefore of interest in future studies.

Place, publisher, year, edition, pages
Hoboken, USA: Wiley-Blackwell, 2015. Vol. 123, no 7, 589-595 p.
Keyword [en]
Staphylococcus epidermidis, ST2, ST215, prosthetic joint infections, airborne transmission
National Category
Infectious Medicine Immunology in the medical area
Research subject
Immunology; Microbiology; Pathology
Identifiers
URN: urn:nbn:se:oru:diva-44704DOI: 10.1111/apm.12392ISI: 000356972400007PubMedID: 25951935Scopus ID: 2-s2.0-84932196136OAI: oai:DiVA.org:oru-44704DiVA: diva2:814627
Note

Funding Agencies:

Örebro County Council Research Committee, Örebro Sweden

Centre for Clinical Research, Västerås

County Council of Västmanland Research Fund

Available from: 2015-05-27 Created: 2015-05-27 Last updated: 2017-10-18Bibliographically approved

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Månsson, EmeliHellmark, BengtSundqvist, MartinSöderquist, Bo
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School of Health and Medical Sciences, Örebro University, SwedenÖrebro University HospitalSchool of Medicine, Örebro University, Sweden
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Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)
Infectious MedicineImmunology in the medical area

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