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Detection of Yersinia enterocolitica in food by PCR amplification
National Food Administration, Swedish University of Agricultural Sciences, Uppsala .
National Food Administration, Swedish University of Agricultural Sciences, Uppsala.
Department of Food Hygiene, Swedish University of Agricultural Sciences, Uppsala.
National Food Administration, Swedish University of Agricultural Sciences, Uppsala.
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1998 (English)In: Letters in Applied Microbiology, ISSN 0266-8254, E-ISSN 1472-765X, Vol. 26, p. 140-144Article in journal (Refereed) Published
Abstract [en]

A polymerase chain reaction (PCR) assay was developed for detection ofpathogenic, virulent strains of Yersinia enterocolitica. By using both virulence loci virFand ail as markers for pathogenicity, detection of species with a virulence factor present waspossible. DNA preparation in the presence of hexadecyl trimethy ammonium bromide(CTAB) was followed by two 44 cycle amplification reactions, one for each of themarkers. As few as 102Y. enterocolitica cells were detected in ground pork in thepresence of 105–106bacteria of other species. The described PCR assay providesa sensitive robust assay for the detection of virulent Y. enterocolitica in food.

Place, publisher, year, edition, pages
Oxon, United Kingdom: Blackwell Publishing, 1998. Vol. 26, p. 140-144
National Category
Medical and Health Sciences Microbiology in the medical area
Research subject
Culinary Arts and Meal Science
Identifiers
URN: urn:nbn:se:oru:diva-45050DOI: 10.1046/j.1472-765X.1998.00296.xISI: 000072683900013PubMedID: 9569698Scopus ID: 2-s2.0-0031980067OAI: oai:DiVA.org:oru-45050DiVA, id: diva2:826752
Available from: 2015-06-25 Created: 2015-06-25 Last updated: 2018-01-11Bibliographically approved

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Danielsson-Tham, Marie-Louise

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