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Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice
Alimentary Pharmabiotic Centre (APC), Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland .
Alimentary Pharmabiotic Centre (APC), Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland.
Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
Alimentary Pharmabiotic Centre (APC), Cork, Ireland. (Nutrition-Gut-Brain Interactions Research Centre)
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2011 (English)In: Microbiology, ISSN 1350-0872, E-ISSN 1465-2080, Vol. 157, no 2, p. 609-615Article in journal (Refereed) Published
Abstract [en]

We have previously demonstrated that oral administration of a metabolically active Bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (CLA), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA and omega-3 (n-3) fatty acids in liver and adipose tissue. In this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production of t10, c12 CLA) from Propionibacterium acnes (PAI) could influence the fatty acid composition of different tissues in a mouse model. Linoleic-acid-supplemented diets (2 %, w/w) were fed in combination with either a recombinant t10, c12 CLA-producing Lactobacillus paracasei NFBC 338 (Lb338), or an isogenic (vector-containing) control strain, to BALB/c mice for 8 weeks. A third group of mice received linoleic acid alone (2 %, w/w). Tissue fatty acid composition was assessed by GLC at the end of the trial. Ingestion of the strain expressing linoleic acid isomerase was associated with a 4-fold increase (P<0.001) in t10, c12 CLA in adipose tissues of the mice when compared with mice that received the isogenic non-CLA-producing strain. The livers of the mice that received the recombinant CLA-producing Lb338 also contained a 2.5-fold (albeit not significantly) higher concentration of t10, c12 CLA, compared to the control group. These data demonstrate that a single gene (encoding linoleic acid isomerase) expressed in an intestinal microbe can influence the fatty acid composition of host fat.

Place, publisher, year, edition, pages
Berks, United Kingdom: Society for General Microbiology , 2011. Vol. 157, no 2, p. 609-615
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Medical and Health Sciences Microbiology
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URN: urn:nbn:se:oru:diva-46363DOI: 10.1099/mic.0.043406-0ISI: 000288483200030PubMedID: 21178166Scopus ID: 2-s2.0-79951500672OAI: oai:DiVA.org:oru-46363DiVA, id: diva2:866260
Available from: 2015-11-02 Created: 2015-11-02 Last updated: 2018-05-07Bibliographically approved

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Wall, Rebecca

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