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Exopolysaccharide-producing probiotic Lactobacilli reduce serum cholesterol and modify enteric microbiota in ApoE-deficient mice
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Teagasc Food Research Centre Moorepark, Fermoy, Ireland.
Centre for Research in Vascular Biology, University College Cork, Cork, Ireland.
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. (Nutrition-Gut-Brain Interactions Research Centre)
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland.
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2014 (English)In: Journal of Nutrition, ISSN 0022-3166, E-ISSN 1541-6100, Vol. 144, no 12Article in journal (Refereed) Published
Abstract [en]

Background: Probiotic bacteria have been associated with a reduction in cardiovascular disease risk, a leading cause of death and disability.

Objectives: The aim of this study was to assess the impact of dietary administration of exopolysaccharide-producing probiotic Lactobacillus cultures on lipid metabolism and gut microbiota in apolipoprotein E (apoE)-deficient mice.

Methods: First, we examined lipid metabolism in response to dietary supplementation with recombinant β-glucan-producing Lactobacillus paracasei National Food Biotechnology Centre (NFBC) 338 expressing the glycosyltransferase (Gtf) gene from Pediococcus parvulus 2.6 (GTF), and naturally exopolysaccharide-producing Lactobacillus mucosae Dairy Product Culture Collection (DPC) 6426 (DPC 6426) compared with the non-β-glucan-producing isogenic control strain Lactobacillus paracasei NFBC 338 (PNZ) and placebo (15% wt:vol trehalose). Second, we examined the effects on the gut microbiota of dietary administration of DPC 6426 compared with placebo. Probiotic Lactobacillus strains at 1 × 10(9) colony-forming units/d per animal were administered to apoE(-/-) mice fed a high-fat (60% fat)/high-cholesterol (2% wt:wt) diet for 12 wk. At the end of the study, aortic plaque development and serum, liver, and fecal variables involved in lipid metabolism were analyzed, and culture-independent microbial analyses of cecal content were performed.

Results: Total cholesterol was reduced in serum (P < 0.001; ∼33-50%) and liver (P < 0.05; ∼30%) and serum triglyceride concentrations were reduced (P < 0.05; ∼15-25%) in mice supplemented with GTF or DPC 6426 compared with the PNZ or placebo group, respectively. In addition, dietary intervention with GTF led to increased amounts of fecal cholesterol excretion (P < 0.05) compared with all other groups. Compositional sequencing of the gut microbiota revealed a greater prevalence of Porphyromonadaceae (P = 0.001) and Prevotellaceae (P = 0.001) in the DPC 6426 group and lower proportions of Clostridiaceae (P < 0.05), Peptococcaceae (P < 0.001), and Staphylococcaceae (P < 0.01) compared with the placebo group.

Conclusion: Ingestion of exopolysaccharide-producing lactobacilli resulted in seemingly favorable improvements in lipid metabolism, which were associated with changes in the gut microbiota of mice.

Place, publisher, year, edition, pages
Bethesda: American Society for Nutrition , 2014. Vol. 144, no 12
Keywords [en]
probiotics, lactobacilli, exopolysaccharide, cholesterol, gut microbiota, lipid metabolism
National Category
Medical and Health Sciences Nutrition and Dietetics
Identifiers
URN: urn:nbn:se:oru:diva-46357DOI: 10.3945/jn.114.191627ISI: 000345199500011PubMedID: 25320181Scopus ID: 2-s2.0-84912036309OAI: oai:DiVA.org:oru-46357DiVA, id: diva2:866264
Available from: 2015-11-02 Created: 2015-11-02 Last updated: 2018-09-16Bibliographically approved

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Wall, Rebecca

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