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Analytical specificity and sensitivity of the novel dual-target GeneProof Neisseria gonorrhoeae PCR kit for detection of N-gonorrhoeae
WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
2015 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 123, no 11, 955-958 p.Article in journal (Refereed) Published
Abstract [en]

Detection of Neisseria gonorrhoeae relies increasingly on nucleic acid amplification tests (NAATs). The specificity of many gonococcal NAATs has been suboptimal and supplementary testing remains recommended in Europe and several additional countries. The novel dual-target GeneProofNeisseria gonorrhoeae PCR kit, targeting porA pseudogene and 16S rRNA gene, showed a high specificity and sensitivity when isolates of non-gonococcal Neisseria and related species (n=144), and gonococci (n=104) were tested. However, rare gonococcal porA mutants were only detected in the 16S rRNA gene target and two non-gonococcal isolates showed a low-level cross-reactivity in the 16S rRNA gene target. The detection limit for both targets was 1.5 copies per reaction.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2015. Vol. 123, no 11, 955-958 p.
Keyword [en]
Gonorrhoea, nucleic acid amplification test, diagnostics, porA pseudogene, 16S rRNA gene
National Category
Immunology in the medical area Microbiology in the medical area
Research subject
Immunology; Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-46695DOI: 10.1111/apm.12440ISI: 000363695900007PubMedID: 26332192Scopus ID: 2-s2.0-84945489846OAI: oai:DiVA.org:oru-46695DiVA: diva2:873256
Available from: 2015-11-23 Created: 2015-11-23 Last updated: 2017-10-17Bibliographically approved

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Hellmark, BengtUnemo, Magnus
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