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A comparison between a PCR method and a conventional culture method for detecting pathogenic Yersinia enterocolitica in food
National Food Administration, Uppsala, Sweden.
National Food Administration, Uppsala, Sweden.
National Food Administration, Uppsala, Sweden.
National Food Administration, Uppsala, Sweden.
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1996 (English)In: Journal of Applied Bacteriology, ISSN 0021-8847, Vol. 81, no 3, 303-308 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to develop a polymerase chain reaction (PCR) method for the detection of pathogenic Yersinia enterocolitica and to compare it with an official culture method (NMKL-117). Primers were selected for nested PCR directed at the attachment invasion locus, ail, on the bacterial chromosome, as well as at a sequence on the pathogenic marker plasmid, termed virulence factor, virF. The final results obtained by the two methods were similar. However, while the conventional method yielded contradictory data for some steps the PCR method provided unambiguous results. Considerable advantages, i.e. higher sensitivity and specificity of the PCR method, compared with the conventional method for detecting pathogenic Y. enterocolitica, were demonstrated in this study.

Place, publisher, year, edition, pages
Blackwell Publishing, 1996. Vol. 81, no 3, 303-308 p.
National Category
Food Science Medical Biotechnology
Research subject
Culinary Arts and Meal Science
Identifiers
URN: urn:nbn:se:oru:diva-46829DOI: 10.1111/j.1365-2672.1996.tb04332.xISI: A1996VG84800011PubMedID: 8810057Scopus ID: 2-s2.0-0029821377OAI: oai:DiVA.org:oru-46829DiVA: diva2:874506
Available from: 2015-11-27 Created: 2015-11-27 Last updated: 2015-12-03Bibliographically approved

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