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The brominated flame retardants TBP-AE and TBP-DBPE antagonize the chicken androgen receptor and act as potential endocrine disrupters in chicken LMH cells
Örebro University, School of Science and Technology.
Biology, The Life Science Center, School of Science and Technology, Örebro University, Örebro, Sweden.ORCID iD: 0000-0003-3302-7106
Örebro University, School of Science and Technology.
Örebro University, School of Science and Technology.
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2015 (English)In: Toxicology in Vitro, ISSN 0887-2333, E-ISSN 1879-3177, Vol. 29, no 8, 1993-2000 p.Article in journal (Refereed) Published
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Text
Abstract [en]

Increased exposure of birds to endocrine disrupting compounds has resulted in developmental and reproductive dysfunctions. We have recently identified the flame retardants, ally1-2,4,6-tribromophenyl ether (TBP-AE), 2-3-dibromopropy1-2,4,6-tribromophenyl ether (TBP-DBPE) and the TBP-DBPE metabolite 2-bromoallyI-2,4,6-tribromophenyl ether (TBP-BAE) as antagonists to both the human androgen receptor (AR) and the zebrafish AR. In the present study, we aimed at determining whether these compounds also interact with the chicken AR. In silico modeling studies showed that TBP-AE, TBP-BAE and TBP-DBPE were able to dock into to the chicken AR ligand-binding pocket. In vitro transfection assays revealed that all three brominated compounds acted as chicken AR antagonists, inhibiting testosterone induced AR activation. In addition, qRT-PCR studies confirmed that they act as AR antagonists and demonstrated that they also alter gene expression patterns of apoptotic, anti-apoptotic, drug metabolizing and amino acid transporter genes. These studies, using chicken LMH cells, suggest that TBP-AE, TBP-BAE and TBP-DBPE are potential endocrine disrupters in chicken.

Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 29, no 8, 1993-2000 p.
Keyword [en]
EDC, Avian, Signaling pathways, ATE, BATE, DPTE, DBE-DBCH, TBECH
National Category
Pharmacology and Toxicology Biological Sciences
Research subject
Biology
Identifiers
URN: urn:nbn:se:oru:diva-47080DOI: 10.1016/j.tiv.2015.08.009ISI: 000364892000004PubMedID: 26318274Scopus ID: 2-s2.0-84940502384OAI: oai:DiVA.org:oru-47080DiVA: diva2:882550
Funder
Swedish Research Council, 20110183
Note

Funding Agency:

Örebro University J61900

Available from: 2015-12-15 Created: 2015-12-15 Last updated: 2017-10-17Bibliographically approved

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