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Separation and determination of neuraminic acids in nanogram quantities by liquid chromatography: A comparative study with different amino phases
Biochemie, Universität des Saarlandes, Saarbrücken, Germany. (MTM Research Centre)ORCID iD: 0000-0003-1496-9245
Biochemie, Universität des Saarlandes, Saarbrücken, Germany.
1985 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 20, no 4, p. 231-234Article in journal (Refereed) Published
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Abstract [en]

Neuraminic acid derivatives were separated easily by liquid chromatography on amino phases and using acetonitrile/15 mM phosphate buffer, pH 5.2 as mobile phase. Detection at 200 nm allowed determination of substances in the nanomole range. All solvent systems were used isocratically at room temperature. The separation did not depend on ion-exchange but on a partition mechanism, the solutes being separated according to their polarity. Thus, it was possible to optimize the resolution by varying the stationary phase and the composition of the eluent.

Place, publisher, year, edition, pages
Springer, 1985. Vol. 20, no 4, p. 231-234
Keywords [en]
Amino silica gel; Column liquid chromatography; Neuraminic acids; UV detection at nanomole level
National Category
Chemical Sciences
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-47673DOI: 10.1007/BF02259693ISI: A1985AHL9700005Scopus ID: 2-s2.0-8544276017OAI: oai:DiVA.org:oru-47673DiVA, id: diva2:896581
Note

Part of this work has been presented at the 15th International Symposium on Chromatography, Nürnberg, October 1–5, 1984.

Available from: 2016-01-21 Created: 2016-01-19 Last updated: 2017-11-30Bibliographically approved

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Fiedler, Heidelore

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