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Genetic diversity of bla(TEM) alleles, antimicrobial susceptibility and molecular epidemiological characteristics of penicillinase-producing Neisseria gonorrhoeae from England and Wales
Sexually Transmitted Bacteria Reference Unit, Public Health England, London, UK.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. WHO Collaborating Centre for Gonorrhoea and other STIs, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology.
Sexually Transmitted Bacteria Reference Unit, Public Health England, London, UK.
National Mycobacterium Reference Laboratory, Public Health England, London, UK.
Show others and affiliations
2015 (English)In: Journal of Antimicrobial Chemotherapy, ISSN 0305-7453, E-ISSN 1460-2091, Vol. 70, no 12, 3238-3243 p.Article in journal (Refereed) Published
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Text
Abstract [en]

Objectives: The objective of this study was to investigate the genetic diversity of bla(TEM) alleles, antimicrobial susceptibility and molecular epidemiological characteristics of penicillinase-producing Neisseria gonorrhoeae (PPNG) isolates collected in 2012 from England and Wales.

Methods: PPNG isolates were from the 2012 Gonococcal Resistance to Antimicrobial Surveillance Programme (GRASP). Their susceptibility to seven antimicrobials was determined using agar dilution methodology. beta-Lactamase production was detected using a nitrocefin test. beta-Lactamase plasmid types were determined and bla(TEM) genes were sequenced. Isolates were also typed by N. gonorrhoeae multi-antigen sequence typing (NG-MAST).

Results: Seventy-three PPNG isolates were identified in the 2012 GRASP collection (4.6%, 73/1603). Three different bla(TEM) alleles were identified, encoding three TEM amino acid sequences: TEM-1 (53%), TEM-1 with a P14S substitution (19%) and TEM-135 (27%). The bla(TEM-135) allele was present in nine different NG-MAST types and was found mostly on Asian (60%) and Toronto/Rio (35%) plasmids. By contrast, most TEM-1-encoding plasmids were African (98%). All the TEM-135 isolates displayed high-level ciprofloxacin and tetracycline resistance.

Conclusions: The high proportion of bla(TEM-135) alleles (27%) demonstrates that this variant is circulating within several gonococcal lineages. Only a single specific mutation near the beta-lactamase active site could result in TEM-135 evolving into an ESBL. This is concerning particularly because the TEM-135 isolates were associated with high-level ciprofloxacin and tetracycline resistance. It is encouraging that no further TEM alleles were detected in this gonococcal population; however, vigilance is vital as an ESBL in N. gonorrhoeae would render the last remaining option for monotherapy, ceftriaxone, useless.

Place, publisher, year, edition, pages
Oxford University Press, 2015. Vol. 70, no 12, 3238-3243 p.
National Category
Infectious Medicine
Research subject
Infectious Diseases
Identifiers
URN: urn:nbn:se:oru:diva-47984DOI: 10.1093/jac/dkv260ISI: 000368246800010PubMedID: 26318193OAI: oai:DiVA.org:oru-47984DiVA: diva2:900829
Note

Funding Agencies:

Achaogen Inc.

Allecra Antiinfectives GmbH

Amplex

AstraZeneca UK Ltd

Becton Dickinson Diagnostics

BSAC

Cepheid

Check-Points B.V.

Cubist Pharmaceuticals

Department of Health

Food Standards Agency

GlaxoSmithKline Services Ltd

Henry Stewart Talks

IHMA Ltd

Merck Sharpe Dohme Corp.

Meiji Seika Kiasya Ltd

Momentum Biosciences Ltd

Nordic Pharma Ltd

Norgine Pharmaceuticals

Rempex Pharmaceuticals Ltd

Rokitan Ltd

Smith Nephew UK Ltd

Trius Therapeutics

VenatoRx

Wockhardt Ltd.

Available from: 2016-02-05 Created: 2016-02-05 Last updated: 2016-02-05Bibliographically approved

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CiteExportLink to record
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