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Ovariectomy and 17β-estradiol replacement in rats and mice: a visual demonstration
Linköping University, Linköping, Sweden.ORCID iD: 0000-0002-6681-0546
Linköping University, Linköping, Sweden.
Linköping University, Linköping, Sweden.
Linköping University, Linköping, Sweden.
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2012 (English)In: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, no 64, e4013Article in journal (Refereed) Published
Abstract [en]

Estrogens are a family of female sexual hormones with an exceptionally wide spectrum of effects. When rats and mice are used in estrogen research they are commonly ovariectomized in order to ablate the rapidly cycling hormone production, replacing the 17β-estradiol exogenously. There is, however, lack of consensus regarding how the hormone should be administered to obtain physiological serum concentrations. This is crucial since the 17β-estradiol level/administration method profoundly influences the experimental results. We have in a series of studies characterized the different modes of 17β-estradiol administration, finding that subcutaneous silastic capsules and per-oral nut-cream Nutella are superior to commercially available slow-release pellets (produced by the company Innovative Research of America) and daily injections in terms of producing physiological serum concentrations of 17β-estradiol. Amongst the advantages of the nut-cream method, that previously has been used for buprenorphine administration, is that when used for estrogen administration it resembles peroral hormone replacement therapy and is non-invasive. The subcutaneous silastic capsules are convenient and produce the most stable serum concentrations. This video article contains step-by-step demonstrations of ovariectomy and 17β-estradiol hormone replacement by silastic capsules and peroral Nutella in rats and mice, followed by a discussion of important aspects of the administration procedures.

Place, publisher, year, edition, pages
Cambridge, USA: Journal of Visualized Experiments , 2012. no 64, e4013
Keyword [en]
17β-estradiol, administration, issue 64, medicine, mouse, nutella, oophorectomy, physiology, rat, silastic capsules
National Category
Medical and Health Sciences Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:oru:diva-48105DOI: 10.3791/4013ISI: 000209223100035PubMedID: 22710371Scopus ID: 2-s2.0-84863746495OAI: oai:DiVA.org:oru-48105DiVA: diva2:901429
Available from: 2016-02-08 Created: 2016-02-08 Last updated: 2017-03-24Bibliographically approved

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Ström, Jakob O.Ingberg, Edvin
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CiteExportLink to record
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Citation style
  • apa
  • harvard1
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  • modern-language-association-8th-edition
  • vancouver
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Language
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