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Glucocorticoids can affect Pseudomonas aeruginosa (ATCC 27853) internalization and intracellular calcium concentration in cystic fibrosis bronchial epithelial cells
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.ORCID iD: 0000-0003-4946-3228
School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
Stockholm CF-Center, Department of Pediatrics, Karolinska University Hospital Huddinge, Stockholm, Sweden.
School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
2015 (English)In: Experimental Lung Research, ISSN 0190-2148, E-ISSN 1521-0499, Vol. 41, no 7, 383-392 p.Article in journal (Refereed) Published
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Text
Abstract [en]

Background and Objective: Glucocorticoids (GCs) are anti-inflammatory agents, but their use in cystic fibrosis (CF) is controversial. In CF, the early colonization with Pseudomonas aeruginosa is mainly due to nonmucoid strains that can internalize, and induce apoptosis in the epithelial cells. Uptake of P. aeruginosa by the epithelial cells and subsequent apoptosis may prevent colonization of P. aeruginosa in CF airways. In the airway epithelia, several other biological effects, including an anti-secretory role by decreasing intracellular Ca2+ concentration have been described for this anti-inflammatory drug. However, the effects of GCs on the nonmucoid P. aeruginosa internalization and intracellular Ca2+ in CF bronchial epithelial cells have not been evaluated.

Methods: We used cultured human CF bronchial airway epithelial cell (CFBE) monolayers to determine P. aeruginosa internalization, apoptosis, and intracellular Ca(2+)concentration in CF bronchial epithelial cells. Cells were treated with IL-6, IL-8, dexamethasone, betamethasone, or budesonide.

Results: GCs in co-treatments with IL-6 reversed the effect of IL-6 by decreasing the internalization of P. aeruginosa in the CFBE cells. GCs decreased the extent of apoptosis in CFBE cells infected with internalized P. aeruginosa, and increased the intracellular Ca2+ concentration.

Conclusion: These findings suggest that if internalization of P. aeruginosa reduces infection, GC therapy would increase the risk of pulmonary infection by decreasing the internalization of P. aeruginosa in CF cells, but GCs may improve airway hydration by increasing the intracellular Ca2+ concentration. Whether the benefits of GC treatment outweigh the negative effects is questionable, and further clinical studies need to be carried out.

Place, publisher, year, edition, pages
Taylor & Francis, 2015. Vol. 41, no 7, 383-392 p.
Keyword [en]
Cystic fibrosis, glucocorticoids, internalization, intracellular Ca2+, Pseudomonas aeruginosa
National Category
Respiratory Medicine and Allergy
Research subject
Pulmonary Medicine
Identifiers
URN: urn:nbn:se:oru:diva-48764DOI: 10.3109/01902148.2015.1046199ISI: 000369738800003PubMedID: 26151838Scopus ID: 2-s2.0-84942123541OAI: oai:DiVA.org:oru-48764DiVA: diva2:907563
Funder
Swedish Heart Lung Foundation
Available from: 2016-02-29 Created: 2016-02-29 Last updated: 2017-10-17Bibliographically approved

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Hussain, RashidaRoomans, Godfried M.
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