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Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication
Institute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark; Department of Biomedical Sciences, Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Institute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark.
Örebro University, School of Health Sciences.ORCID iD: 0000-0002-9831-0896
Institute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark.
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2016 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 30, no 6, 2266-2281 p.Article in journal (Refereed) Published
Abstract [en]

With this study we investigated the role of nonsteroidal anti-inflammatory drugs (NSAIDs) in human skeletal muscle regeneration. Young men ingested NSAID [1200 mg/d ibuprofen (IBU)] or placebo (PLA) daily for 2 wk before and 4 wk after an electrical stimulation-induced injury to the leg extensor muscles of one leg. Muscle biopsies were collected from the vastus lateralis muscles before and after stimulation (2.5 h and 2, 7, and 30 d) and were assessed for satellite cells and regeneration by immunohistochemistry and real-time RT-PCR, and we also measured telomere length. After injury, and compared with PLA, IBU was found to augment the proportion of ActiveNotch1(+) satellite cells at 2 d [IBU, 29 ± 3% vs. PLA, 19 ± 2% (means ± sem)], satellite cell content at 7 d [IBU, 0.16 ± 0.01 vs. PLA, 0.12 ± 0.01 (Pax7(+) cells/fiber)], and to expedite muscle repair at 30 d. The PLA group displayed a greater proportion of embryonic myosin(+) fibers and a residual ∼2-fold increase in mRNA levels of matrix proteins (all P < 0.05). Endomysial collagen was also elevated with PLA at 30 d. Minimum telomere length shortening was not observed. In conclusion, ingestion of NSAID has a potentiating effect on Notch activation of satellite cells and muscle remodeling during large-scale regeneration of injured human skeletal muscle.-Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., Aagaard, P., Durigan, J. L. Q., Kjaer, M. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication.

Place, publisher, year, edition, pages
Bethesda, USA: Federation of American Societies for Experimental Biology , 2016. Vol. 30, no 6, 2266-2281 p.
Keyword [en]
Pax7, electrical stimulation, telomere length, extracellular matrix, Notch signaling pathway
National Category
Physiology Cell and Molecular Biology
Research subject
Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-49204DOI: 10.1096/fj.201500198RISI: 000376878300018PubMedID: 26936358Scopus ID: 2-s2.0-84971654983OAI: oai:DiVA.org:oru-49204DiVA: diva2:911296
Note

Funding Agencies:

U.S. National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Nordea Foundation 223576

European Commission

Danish Rheumatism Association

Team Danmark

Anti Doping Danmark

Oster-Jorgensen og Romhild Andersen Fonden

King Christian IX and Queen Louise's Anniversary grant (Kong Christian IX og Dronning Louises Jubilaeumslegat)

Danish Agency for Science Technology and Innovation (Medical Research Council)

Lundbeck Foundation

Available from: 2016-03-11 Created: 2016-03-10 Last updated: 2016-07-26Bibliographically approved

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