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Transcriptional profiling of human smooth muscle cells infected with gingipain and fimbriae mutants of Porphyromonas gingivalis
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
Örebro University, School of Medicine, Örebro University, Sweden. Univ Orebro, Sch Hlth Sci, Dept Clin Med, SE-70182 Orebro, Sweden..ORCID iD: 0000-0002-0278-4510
Örebro University, School of Medicine, Örebro University, Sweden.
Örebro University, School of Medicine, Örebro University, Sweden.
2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 21911Article in journal (Refereed) Published
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Abstract [en]

Porphyromonas gingivalis (P. gingivalis) is considered to be involved in the development of atherosclerosis. However, the role of different virulence factors produced by P. gingivalis in this process is still uncertain. The aim of this study was to investigate the transcriptional profiling of human aortic smooth muscle cells (AoSMCs) infected with wild type, gingipain mutants or fimbriae mutants of P. gingivalis. AoSMCs were exposed to wild type (W50 and 381), gingipain mutants (E8 and K1A), or fimbriae mutants (DPG-3 and KRX-178) of P. gingivalis. We observed that wild type P. gingivalis changes the expression of a considerable larger number of genes in AoSMCs compare to gingipain and fimbriae mutants, respectively. The results from pathway analysis revealed that the common differentially expressed genes for AoSMCs infected by 3 different wild type P. gingivalis strains were enriched in pathways of cancer, cytokine-cytokine receptor interaction, regulation of the actin cytoskeleton, focal adhesion, and MAPK signaling pathway. Disease ontology analysis showed that various strains of P. gingivalis were associated with different disease profilings. Our results suggest that gingipains and fimbriae, especially arginine-specific gingipain, produced by P. gingivalis play important roles in the association between periodontitis and other inflammatory diseases, including atherosclerosis.

Place, publisher, year, edition, pages
London United Kingdom: Nature Publishing Group, 2016. Vol. 6, 21911
National Category
Cell and Molecular Biology
Research subject
Cell Research
Identifiers
URN: urn:nbn:se:oru:diva-49361DOI: 10.1038/srep21911ISI: 000370717700001PubMedID: 26907358Scopus ID: 2-s2.0-84959377942OAI: oai:DiVA.org:oru-49361DiVA: diva2:912461
Funder
Swedish Heart Lung Foundation, T77414Knowledge Foundation
Note

Funding Agencies:

Foundation of Olle Engkvist

Foundation of Mats Kleberg

Available from: 2016-03-16 Created: 2016-03-16 Last updated: 2017-10-18Bibliographically approved

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