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Division of Listeria monocytogenes serovar 4b strains into two groups by PCR and restriction enzyme analysis
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.ORCID iD: 0000-0003-0154-9452
Medical Bacteriology Laboratory, University Hospital, Lausanne, Switzerland.
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1995 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 61, no 11, 3872-3874 p.Article in journal (Refereed) Published
Abstract [en]

Altogether, 133 strains of Listeria monocytogenes serovar 4b were investigated, A segment of 2,916 bp containing parts of the two genes inlA and inlB in L. monocytogenes was amplified by the PCR technique. The PCR product obtained was cleaved with the restriction enzyme AluI, and the fragments generated were separated by gel electrophoresis, leading to two distinct groups: PCR-restriction enzyme analysis groups I and II, containing 37 and 96 strains, respectively, The PCR-restriction enzyme analysis method described in this paper could be a useful tool for the subtyping of L. monocytogenes serovar 4b strains.

Place, publisher, year, edition, pages
American Society for Microbiology , 1995. Vol. 61, no 11, 3872-3874 p.
National Category
Food Science Microbiology
Research subject
Culinary Arts and Meal Science
Identifiers
URN: urn:nbn:se:oru:diva-49485ISI: A1995TC81100017PubMedID: 8526498Scopus ID: 2-s2.0-0028805166OAI: oai:DiVA.org:oru-49485DiVA: diva2:914784
Available from: 2016-03-25 Created: 2016-03-25 Last updated: 2017-10-17Bibliographically approved

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