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Evaluation of HPV Genotyping Assays for Archival Clinical Samples
Örebro University Hospital. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
Örebro University Hospital. Department of Urology, Örebro University Hospital, Örebro, Sweden.ORCID iD: 0000-0001-5533-7899
Örebro University Hospital. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.ORCID iD: 0000-0001-6881-237X
Örebro University Hospital. Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.ORCID iD: 0000-0003-2317-5738
2015 (English)In: Journal of Molecular Diagnostics, ISSN 1525-1578, E-ISSN 1943-7811, Vol. 17, no 3, p. 293-301Article in journal (Refereed) Published
Abstract [en]

Human papillomavirus (HPV) testing and genotyping of FFPE tissue samples is important in epidemiological investigations. Here, we compare four different HPV genotyping methods for use in FFPE clinical samples. Comparative testing was performed on 99 samples with a clinical suspicion of HPV. Specimens were analyzed with Anyplex II HPV28 detecting 28 genotypes using real-time PCR and melting curve analysis, CLART HPV2 detecting 35 genotypes using PCR and microarray detection, and MGP5+/6+ consensus primer system together with pyrosequencing. Results were compared to a real-time PCR reference protocol detecting 14 genotypes. In total, 68% of the samples were positive for an HPV genotype using the reference protocol and MGP5+/6+ primer system. Anyplex II HPV28 analysis and CLART HPV2 had 82% and 72% positive samples, respectively. All four methods showed good agreement when comparing the 14 genotypes included in the reference protocol. When evaluating all genotypes, the Anyplex II HPV28 assay and the CLART assay changed the status of the sample (individually or together) from negative with respect to the reference protocol to positive for either a Group 1 (n = 4) or Group 2 (n = 6) genotype. We conclude from this study that for an extended genotyping approach with a high sensitivity for FFPE specimens, both the Anyplex II HPV28 and CLART HPV2 assays are suitable alternatives despite minor intra-assay differences.

Place, publisher, year, edition, pages
New York, USA: Elsevier, 2015. Vol. 17, no 3, p. 293-301
National Category
Clinical Laboratory Medicine Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:oru:diva-49487DOI: 10.1016/j.jmoldx.2014.12.004ISI: 000353843900009PubMedID: 25791291Scopus ID: 2-s2.0-84928158512OAI: oai:DiVA.org:oru-49487DiVA, id: diva2:914918
Available from: 2016-03-27 Created: 2016-03-25 Last updated: 2018-07-03Bibliographically approved

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Lillsunde-Larsson, GabriellaCarlsson, JessicaKarlsson, Mats G.Helenius, Gisela

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