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Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
Örebro University, School of Health Sciences. iRiSC - Inflammatory Responses and Infection Susceptibility Centre.
Örebro University, School of Medical Sciences. iRiSC - Inflammatory Responses and Infection Susceptibility Centre.
Örebro University, School of Medical Sciences.
Örebro University, School of Medical Sciences. iRiSC - Inflammatory Responses and Infection Susceptibility Centre.
2016 (English)In: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 16, no 1, 64Article in journal (Refereed) Published
Abstract [en]

Background: Increased resistance to antimicrobial agents is a characteristic of many bacteria growing in biofilms on for example indwelling urinary catheters or in intracellular bacterial reservoirs. Biofilm-related infections caused by multidrug-resistant bacteria, such as extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, are a major challenge. The aim of this study was to investigate if a carbon monoxide-releasing molecule (CORM-2) has antibacterial effects against ESBL-producing uropathogenic E. coli (UPEC) in the biofilm mode of growth and following colonization of host bladder epithelial cells.

Results: The effect of CORM-2 was examined on bacteria grown within an established biofilm (biofilm formed for 24 h on plastic surface) by a live/dead viability staining assay. CORM-2 (500 μM) exposure for 24 h killed approximately 60 % of the ESBL-producing UPEC isolate. A non-ESBL-producing UPEC isolate and the E. coli K-12 strain TG1 were also sensitive to CORM-2 exposure when grown in biofilms. The antibacterial effect of CORM-2 on planktonic bacteria was reduced and delayed in the stationary growth phase compared to the exponential growth phase. In human bladder epithelial cell colonization experiments, CORM-2 exposure for 4 h significantly reduced the bacterial counts of an ESBL-producing UPEC isolate.

Conclusion: This study shows that CORM-2 has antibacterial properties against multidrug-resistant UPEC under biofilm-like conditions and following host cell colonization, which motivate further studies of its therapeutic potential.

Place, publisher, year, edition, pages
London, United Kingdom: BioMed Central, 2016. Vol. 16, no 1, 64
Keyword [en]
Carbon monoxide releasing molecule, CORM-2, extended-spectrum β-lactamase, uropathogenic escherichia coli, biofilm
National Category
Microbiology
Research subject
Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-49877DOI: 10.1186/s12866-016-0678-7ISI: 000374282400001PubMedID: 27067266Scopus ID: 2-s2.0-84964033699OAI: oai:DiVA.org:oru-49877DiVA: diva2:920907
Note

Funding Agencies:

Swedish Council for Working Life and Social Research (FAS)

Faculty of Medicine and Health at Orebro University

Nyckelfonden at Orebro University Hospital

Available from: 2016-04-19 Created: 2016-04-19 Last updated: 2016-05-19Bibliographically approved

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