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Water-in-Oil microemulsions versus emulsions as carriers of hydroxytyrosol: an in vitro gastrointestinal lipolysis study using the pHstat technique
Örebro University, School of Science and Technology. National Hellenic Research Foundation Institute of Biology Medicinal Chemistry and Biotechnology.
CNRS, UMR7282 Enzymologie Interfaciale et Physiologie de la Lipolyse.
Polytechnic Institute of Bourdeaux, CBMN.
National Hellenic Research Foundation Institute of Biology Medicinal Chemistry and Biotechnology.
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(English)Manuscript (preprint) (Other academic)
National Category
Other Chemistry Topics
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-50119OAI: oai:DiVA.org:oru-50119DiVA, id: diva2:925796
Available from: 2016-05-04 Created: 2016-05-03 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Formulation and characterization of W/O nano-dispersions for bioactive delivery applications
Open this publication in new window or tab >>Formulation and characterization of W/O nano-dispersions for bioactive delivery applications
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The main objective of this study was the formulation of food-grade water-in-oil (W/O) nano-dispersions based mainly on medium or long-chain triglycerides. Two types of dispersions were formulated and structurally compared, namely emulsions and microemulsions. The systems were used as matrices for encapsulating targeted bioactive molecules with specific characteristics such as antioxidants or peptides.

The structural characterization of the formulated systems was investigated using techniques such as Electron Paramagnetic Resonance (EPR) spectroscopy, Dynamic Light Scattering (DLS), Cryogenic Transmission Electron Microscopy (Cryo-TEM) and Small Angle Xray Scattering (SAXS). The existence of swollen inverse micelles was revealed for the case of microemulsions whereas larger droplets still at the nano-scale were observed for the case of emulsions. Structural differences in the presence of the bioactive molecules or induced by the alteration of components were also observed.

In order to study the efficacy of the formulations, the proposed loaded systems were assessed either using EPR spectroscopy or Well Diffusion Assay (WDA) depending on the bioactive molecule. It was found that the encapsulated molecules retained their claimed characteristics when encapsulated to the proposed matrices.

Finally, some of the formulated dispersions were investigated for their behavior under gastrointestinal (GI) conditions. A two-step digestion model using recombinant Dog Gastric Lipase (rDGL) and Porcine Pancreatic Lipase (PPL) was proposed to simulate lipid hydrolysis in humans. The studies revealed significant decrease of the rDGL specific activity in the presence of the microemulsion while in the presence of lower percent of surfactants (case of emulsion) no alterations were observed.

Place, publisher, year, edition, pages
Örebro: Örebro university, 2016. p. 77
Series
Örebro Studies in Chemistry, ISSN 1651-4270 ; 16
Keywords
nano-dispersions, encapsulation, food, DLS, EPR, Cryo-TEM, SAXS, pH-stat, digestion, antioxidants, gastric lipase, pancreatic lipase
National Category
Other Chemistry Topics
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-49454 (URN)978-91-7529-141-3 (ISBN)
Public defence
2016-05-26, Billbergska huset, hörsal B, Örebro universitet, Fakultetsgatan 1, Örebro, 10:15 (English)
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Supervisors
Available from: 2016-03-22 Created: 2016-03-22 Last updated: 2017-10-17Bibliographically approved

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