To Örebro University

Plants as well as other aerobic organisms constantly produce reactive oxygen species (ROS). At regulatedlow concentrations ROS may serve as signal molecules, while in excessive amounts these may causeoxidative damage to biomolecules. Actual cellular concentrations are controlled by a network of variousantioxidants, and acclimation to stress conditions is achieved by a dynamic balance of ROS productionand neutralization. Accordingly, plant stress physiology studies generally include an array of methodstesting the occurrence of ROS as well as evaluating antioxidant capacities. The aim of the present workis to provide an overview of these methods, with special emphasis on avoiding errors that can possiblylead to either inaccurate data or misinterpretations of otherwise correct measurements.