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Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Center for Coastal Pollution and Conservation, Department of Biology and Chemistry, City University of Hong Kong, Hong Kong.
Center for Coastal Pollution and Conservation, Department of Biology and Chemistry, City University of Hong Kong, Hong Kong.
Center for Coastal Pollution and Conservation, Department of Biology and Chemistry, City University of Hong Kong, Hong Kong.
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2007 (English)In: Environmental Toxicology and Chemistry, ISSN 0730-7268, E-ISSN 1552-8618, Vol. 26, no 4, p. 764-772Article in journal (Refereed) Published
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Abstract [en]

Brominated flame retardants (BFRs) and brominated dioxins are emerging persistent organic pollutants that are ubiquitous in the environment and can be accumulated by wildlife and humans. These chemicals can disturb endocrine function. Recent studies have demonstrated that one of the mechanisms of endocrine disruption by chemicals is modulation of steroidogenic gene expression or enzyme activities. In this study, an in vitro assay based on the H295R human adrenocortical carcinoma cell line, which possesses most key genes or enzymes involved in steroidogenesis, was used to examine the effects of five bromophenols, two polybrominated biphenyls (PBBs 77 and 169), 2,3,7,8-tetrabromodibenzo-p-dioxin, and 2,3,7,8-tetrabromodibenzofuran on the expression of 10 key steroidogenic genes. The H295R cells were exposed to various BFR concentrations for 48 h, and the expression of specific genes - cytochrome P450 (CYP11A, CYP11B2, CYP17, CYP19, and CYP21), 3β- hydroxysteroid dehydrogenase (3βHSD2), 17β-hydroxysteroid dehydrogenase (17βHSD1 and 17βHSD4), steroidogenic acute regulatory protein (StAR), and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) - was quantitatively measured using real-time polymerase chain reaction. Cell viability was not affected at the doses tested. Most of the genes were either up- or down-regulated, to some extent, by BFR exposure. Among the genes tested, 3βHSD2 was the most markedly up-regulated, with a range of magnitude from 1.6- to 20-fold. The results demonstrate that bromophenol, bromobiphenyls, and bromodibenzo-p-dioxin/furan are able to modulate steroidogenic gene expression, which may lead to endocrine disruption.

Place, publisher, year, edition, pages
Society of Environmental Toxicology and Chemistry (SETAC), 2007. Vol. 26, no 4, p. 764-772
Keywords [en]
Brominated flame retardants; Bromodioxins; Gene expression; H295R; Steroidogenesis
National Category
Environmental Sciences
Research subject
Enviromental Science
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URN: urn:nbn:se:oru:diva-50007DOI: 10.1897/06-388R1.1ISI: 000244938200023PubMedID: 17447562Scopus ID: 2-s2.0-34247530702OAI: oai:DiVA.org:oru-50007DiVA, id: diva2:950392
Available from: 2016-07-29 Created: 2016-04-28 Last updated: 2017-11-28Bibliographically approved

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Yeung, Leo W. Y.Lam, Paul K. S.

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