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Differential expression of chicken hepatic genes responsive to PFOA and PFOS
Safety Research Team, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki, Japan; Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong.ORCID iD: 0000-0001-6800-5658
Safety Research Team, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki, Japan.
Safety Research Team, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki, Japan.
Safety Research Team, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki, Japan.
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2007 (English)In: Toxicology, ISSN 0300-483X, E-ISSN 1879-3185, Vol. 237, no 1-3, p. 111-125Article in journal (Refereed) Published
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Abstract [en]

The effects of PFOS and PFOA on the gene expression patterns of chickens that were exposed to either PFOS or PFOA at low doses were investigated with the use of microarray techniques. Twelve Genechip Chicken Genome Arrays were used to study hepatic gene expression in 6-week-old chickens (Gallus gallus) that were exposed to either PFOA (0.1, 0.5, or 5 mg/mL), PFOS (0.02 or 0.1 mg/mL), or a saline vehicle control (0.9% NaCl in Milli-Q water) via subcutaneous implantation of a 2 mL osmotic pump for 4 weeks or for 4 weeks with a further 4 weeks of depuration. Over 240 and 480 genes were significantly affected by PFOS after 4 weeks of exposure and after 4 weeks of exposure with a further 4 weeks of depuration, respectively and over 290 and 320 genes were significantly affected by PFOA, correspondingly. For PFOS, the genes that were affected after 4 weeks of exposure were mainly related to the transport of electrons and oxygen, and the metabolism of lipids and fatty acids; while the genes that were affected after 4 weeks of exposure with a further 4 weeks of depuration were mainly related to the transport of electrons and ions, and protein amino acid phosphorylation and proteolysis. For PFOA, the genes that were affected after 4 weeks of exposure were related to the transport of ions, lipids, and electrons and cytochromes; while the genes that were affected after 4 weeks of exposure with a further 4 weeks of depuration were related to protein amino acid phosphorylation and proteolysis, the transport of ions, and the metabolism of fatty acids and lipids. The results also showed that the gene expression patterns between chickens that were treated with PFOS and those that were treated with PFOA were different, which points to the importance of the separate evaluation of the toxicities of PFOS and PFOA. Specifically, the gene expressions of CYP8B and NOV were studied.

Place, publisher, year, edition, pages
Elsevier, 2007. Vol. 237, no 1-3, p. 111-125
Keywords [en]
Chicken Genome Array; Depuration; Gene expression; Perfluorooctane sulfonate; Perflurooctanoic acid; Subcutaneous implantation
National Category
Environmental Sciences
Research subject
Enviromental Science
Identifiers
URN: urn:nbn:se:oru:diva-50006DOI: 10.1016/j.tox.2007.05.004ISI: 000248743200011PubMedID: 17560707Scopus ID: 2-s2.0-34447093902OAI: oai:DiVA.org:oru-50006DiVA, id: diva2:950396
Available from: 2016-07-29 Created: 2016-04-28 Last updated: 2017-11-28Bibliographically approved

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Yeung, Leo W. Y.Lam, Paul K. S.

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