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The novel 2016 WHO Neisseria gonorrhoeae reference strains for global quality assurance of laboratory investigations: phenotypic, genetic and reference genome characterization
Örebro University, School of Health Sciences. WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology.
Örebro University, School of Medical Sciences. WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology.
Pathogen Genomics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridgeshire, UK.
Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA; Division of Infectious Diseases Brigham andWomen’s Hospital, Harvard Medical School, Boston, MA, USA.
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2016 (English)In: Journal of Antimicrobial Chemotherapy, ISSN 0305-7453, E-ISSN 1460-2091, Vol. 71, no 11, 3096-3108 p.Article in journal (Refereed) Published
Abstract [en]

Objectives: Gonorrhoea and MDR Neisseria gonorrhoeae remain public health concerns globally. Enhanced, quality-assured, gonococcal antimicrobial resistance (AMR) surveillance is essential worldwide. The WHO global Gonococcal Antimicrobial Surveillance Programme (GASP) was relaunched in 2009. We describe the phenotypic, genetic and reference genome characteristics of the 2016 WHO gonococcal reference strains intended for quality assurance in the WHO global GASP, other GASPs, diagnostics and research worldwide.

Methods: The 2016 WHO reference strains (n = 14) constitute the eight 2008 WHO reference strains and six novel strains. The novel strains represent low-level to high-level cephalosporin resistance, high-level azithromycin resistance and a porA mutant. All strains were comprehensively characterized for antibiogram (n = 23), serovar, prolyliminopeptidase, plasmid types, molecular AMR determinants, N. gonorrhoeae multiantigen sequence typing STs and MLST STs. Complete reference genomes were produced using single-molecule PacBio sequencing.

Results: The reference strains represented all available phenotypes, susceptible and resistant, to antimicrobials previously and currently used or considered for future use in gonorrhoea treatment. All corresponding resistance genotypes and molecular epidemiological types were described. Fully characterized, annotated and finished references genomes (n = 14) were presented.

Conclusions: The 2016 WHO gonococcal reference strains are intended for internal and external quality assurance and quality control in laboratory investigations, particularly in the WHO global GASP and other GASPs, but also in phenotypic (e.g. culture, species determination) and molecular diagnostics, molecular AMR detection, molecular epidemiology and as fully characterized, annotated and finished reference genomes in WGS analysis, transcriptomics, proteomics and other molecular technologies and data analysis.

Place, publisher, year, edition, pages
Oxford, United Kingdom: Oxford University Press, 2016. Vol. 71, no 11, 3096-3108 p.
National Category
Microbiology Infectious Medicine
Research subject
Microbiology; Infectious Diseases
Identifiers
URN: urn:nbn:se:oru:diva-51479DOI: 10.1093/jac/dkw288ISI: 000388005100014PubMedID: 27432602Scopus ID: 2-s2.0-84994481826OAI: oai:DiVA.org:oru-51479DiVA: diva2:951114
Note

Funding Agencies:

Department of Reproductive Health and Research

WHO Headquarters, Geneva, Switzerland

Australian Government Department of Health

Department of Microbiology, South Eastern Area Laboratory Services, New South Wales, Australia

Örebro County Council Research Committee

Foundation for Medical Research at Orebro University Hospital, Sweden

Wellcome Trust 098051

National Institutes of Health/National Institute of Allergy and Infectious Diseases K08-AI104767

Available from: 2016-08-05 Created: 2016-08-02 Last updated: 2017-10-17Bibliographically approved

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