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Antimicrobial resistance and Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) genotypes in N. gonorrhoeae during 2012-2014 in Karachi, Pakistan
Department of Pathology and Laboratory Medicine, Aga Khan University, Stadium Road, Karachi, Pakistan.
Department of Pathology and Laboratory Medicine, Aga Khan University, Stadium Road, Karachi, Pakistan.
Department of Pathology and Laboratory Medicine, Aga Khan University, Stadium Road, Karachi, Pakistan.
Department of Pathology and Laboratory Medicine, Aga Khan University, Stadium Road, Karachi, Pakistan.
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2016 (English)In: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 16, no 1, article id 353Article in journal (Refereed) Published
Abstract [en]

Background: Globally, increasing antimicrobial resistance (AMR) in Neisseria gonorrhoea has led to decreased treatment options for gonorrhoea. Continuous monitoring of resistance is crucial to determine evolving resistance trends in Neisseria gonorrhoea and to suggest treatment recommendations. Quality assured gonococcal AMR data from Pakistan are mainly lacking. This study was performed to determine prevalence and trends of gonococcal AMR and molecular epidemiology of local strains during 2012-2014 in Karachi, Pakistan.

Methods: Gonococcal isolates (n = 100) were obtained from urogenital specimens submitted to the Aga Khan University Laboratory, Karachi, Pakistan. Antimicrobial susceptibility was determined using Etest and molecular epidemiology was assessed by N. gonorrhoeae multiantigen sequence typing (NG-MAST). Quality control was performed using N. gonorrhoeae WHO reference strains C, F, G, K, L, M, N, O, and P, and ATCC 49226.

Results: Susceptibility to spectinomycin, ceftriaxone and cefixime was 100 % and to azithromycin was 99 %. All isolates had low ceftriaxone MICs, i.e., ≤0.032 mg/L. Resistance to ciprofloxacin, tetracycline and penicillin G were 86 %, 51 % and 43 %, respectively. NG-MAST analysis identified 74 different sequence types (STs).

Conclusions: A highly diversified gonococcal population, 74 NG-MAST STs (62 novel STs) with an increased resistance to penicillin G, ciprofloxacin and tetracycline circulated in Karachi, Pakistan. Fortunately, no resistance to ceftriaxone was detected. Accordingly, ceftriaxone can continuously be recommended as the treatment of choice. However it is recommended to increase the dose of ceftriaxone from 125 mg intramuscularly to 250 mg intramuscularly due to ceftriaxone MIC creep and emerging resistance reported in the region. Furthermore, due to the high level of resistance to ciprofloxacin (86 %) it is essential to exclude ciprofloxacin from the recommended first-line therapy. It is imperative to significantly broaden the gonococcal AMR monitoring with participation from other laboratories and cities in Pakistan.

Place, publisher, year, edition, pages
London, United Kingdom: BioMed Central, 2016. Vol. 16, no 1, article id 353
Keywords [en]
Neisseria gonorrhoeae, Gonorrhoea, Antimicrobial resistance, Antimicrobial resistance surveillance, Extended-spectrum cephalosporins (ESCs), Ceftriaxone, Treatment, N. gonorrhoeae multiantigen sequence typing (NG-MAST), Pakistan
National Category
Infectious Medicine
Research subject
Infectious Diseases
Identifiers
URN: urn:nbn:se:oru:diva-51477DOI: 10.1186/s12879-016-1673-1ISI: 000380994900001PubMedID: 27449234Scopus ID: 2-s2.0-84979009565OAI: oai:DiVA.org:oru-51477DiVA, id: diva2:951115
Note

Funding Agencies:

Örebro County Council Research Committee

Foundation for Medical Research at Orebro University Hospital, Sweden

Available from: 2016-08-05 Created: 2016-08-02 Last updated: 2017-11-28Bibliographically approved

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Jacobsson, SusanneUnemo, Magnus

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