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Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Institute of Biology & Medical Genetics, 1st Medical Faculty, Charles University, Prague, Czech Republic.
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Institute of Biology & Medical Genetics, 1st Medical Faculty, Charles University, Prague, Czech Republic; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Czech Republic.
Human Genetics Foundation, (HuGeF), Torino, Italy.
Human Genetics Foundation, (HuGeF), Torino, Italy.
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2016 (English)In: Epigenomics, ISSN 1750-1911, Vol. 8, no 9, 1193-1207 p.Article in journal (Refereed) Published
Abstract [en]

Aim: The aim of the present study is to address a genome-wide search for novel methylation biomarkers in the rectal cancer (RC), as only scarce information on methylation profile is available.

Materials and methods: We analyzed methylation status in 25 pairs of RC and adjacent healthy mucosa using the Illumina Human Methylation 450 BeadChip.

Results: We found significantly aberrant methylation in 33 genes. After validation of our results by pyrosequencing, we found a good agreement with our findings. The BPIL3 and HBBP1 genes resulted hypomethylated in RC, whereas TIFPI2, ADHFE1, FLI1 and TLX1 were hypermethylated. An external validation by TCGA datasets confirmed the results.

Conclusion: Our study, with external validation, has demonstrated the feasibility of using specific methylated DNA signatures for developing biomarkers in RC.

Place, publisher, year, edition, pages
London, United Kingdom: Future Medicine Ltd. , 2016. Vol. 8, no 9, 1193-1207 p.
Keyword [en]
DNA methylation, Illumina Human Methylation 450 BeadChip, rectal cancer
National Category
Cancer and Oncology Medical Genetics
Identifiers
URN: urn:nbn:se:oru:diva-51745DOI: 10.2217/epi-2016-0044ISI: 000383180400005PubMedID: 27529132Scopus ID: 2-s2.0-84986910214OAI: oai:DiVA.org:oru-51745DiVA: diva2:954668
Note

Funding Agencies:

Internal Grant Agency of the Czech Ministry of Health NT 13424  NT/14329-3

Czech Science Foundation 15-27580A  GA15-08239S

COST LD14050

European Regional Development Fund CZ.1.05/2.1.00/03.0076

Lions Cancer Foundation

Nyckelfonden, Örebro lans landstig

Ministry of Education Youth and Sports of the Czech Republic LO1503

Available from: 2016-08-23 Created: 2016-08-23 Last updated: 2016-10-10Bibliographically approved

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