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Analysis of the AhR-inducing Potency and Mutagenicity in a PAH-contaminated Soil during Remediation - Using H4IIE-luc Assay and Ames Fluctuation Test
Örebro University, School of Science and Technology.
2016 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

About 80 000 places in Sweden are considered highly polluted according to the Swedish EPA. The pollutants by which the areas are contaminated can be oil, hazardous chemicals such as persistent organic pollutants or heavy metals. Polycyclic aromatic hydrocarbons (PAHs) is a group of organic pollutants that has proven to present a major health risk, especially after having shown mutagenic effects. This study was a part of the SOILTOX- project at the MTM department of Örebro University. The study involved analysis of an oil- and PAH contaminated soil undergoing bioremediation with focus on PAHs. Previous studies have shown that the AhR- induced toxicity of PAH- soils can increase during remediation, suggesting that it is due to derivatives that are produced during metabolism of the chemicals, or the fact that PAHs become more bioavailable when they are released from the soil particles. Using pressurized liquid extraction (PLE), H4IIE-luc assay and Ames fluctuation test, this study showed that the AhR- mediated toxicity of the soil did not decrease during remediation and that this particular soil was not mutagenic. The results agreed with previous studies in that a decrease in PAH- concentration was not accompanied by a decrease in AhR- agonists, as measured using the H4IIE- luc assay. This shows that there are other chemicals and/or synergistic effects in the matrix to take into account in the bioremediation process.

Abstract [sv]

Omkring 80 000 områden i Sverige anses vara allvarligt förorenade enligt naturvårdsverket. Föroreningarna på dessa områden kan vara olja, giftiga kemikalier som till exempel persistenta organiska föroreningar eller tungmetaller. Polycykliska aromatiska kolväten (PAH) är en grupp organiska föroreningar som har visat sig utgöra en stor hälsorisk, särskilt med tanke på att vissa är mutagena. Denna studie är en del av SOILTOX- projektet vid forskningscentrum MTM (Människa, teknik, miljö) på Örebro universitet. Studien innehöll analys av en olje- och PAH- kontaminerad jord under pågående biosanering, med fokus på PAHer. Tidigare studier har visat att toxiciteten hos PAH- jordar kan öka under sanering. Teorier om vad som ligger bakom detta fenomen är att det bildas metaboliter under nedbrytningen av PAHer, eller att föreningarna blir mer biotillgängliga när de under saneringen "lossnar" från jordpartiklarna och att nedbrytningen av kemikalierna skulle vara långsammare än "frisättningen". Med hjälp av PLE (trycksatt vätskeextraktion), bioanalys i form av H4IIE-luc, samt Ames fluktuationstest, så visade denna studie att den AhR- medierade toxiciteten i jorden inte minskade under sanering, samt att den aktuella jorden inte var mutagen. Resultaten stämde överens med tidigare studier i det avseendet att en minskning i koncentration av PAHer inte innebar en minskning av AhR- agonister, vilket mäts med testet H4IIE- luc. Detta visar att det finns fler kemikalier och/eller synergistiska effekter i matrisen som man måste ta hänsyn till vid biosanering.

Place, publisher, year, edition, pages
2016.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:oru:diva-51764OAI: oai:DiVA.org:oru-51764DiVA: diva2:954790
Subject / course
Biology
Supervisors
Examiners
Available from: 2016-08-23 Created: 2016-08-23 Last updated: 2017-10-17Bibliographically approved

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