oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Bioassay battery interlaboratory investigation of emerging contaminants in spikedwater extracts: Towards the implementation of bioanalytical monitoring tools inwater quality assessment and monitoring
Institute for Environmental Research, RWTH Aachen University, Aachen, Germany.
Institute for Environmental Research, RWTH Aachen University, Aachen, GermanyWTH Aachen University, Aachen, Germany.
Örebro University, School of Science and Technology. Institute for Environmental Research, RWTH Aachen University, Aachen, Germany. (MTM)ORCID iD: 0000-0002-2356-6686
INERIS, Verneuil-en-Halatte, France.
Show others and affiliations
2016 (English)In: Water Research, ISSN 0043-1354, Vol. 104, p. 473-484Article in journal (Refereed) Published
Abstract [en]

Bioassays are particularly useful tools to link the chemical and ecological assessments in water quality monitoring. Different methods cover a broad range of toxicity mechanisms in diverse organisms, and account for risks posed by non-target compounds and mixtures. Many tests are already applied in chemical and waste assessments, and stakeholders from the science-police interface have recommended their integration in regulatory water quality monitoring. Still, there is a need to address bioassay suitability to evaluate water samples containing emerging pollutants, which are a current priority in water quality monitoring. The presented interlaboratory study (ILS) verified whether a battery of miniaturized bioassays, conducted in 11 different laboratories following their own protocols, would produce comparable results when applied to evaluate blinded samples consisting of a pristine water extract spiked with four emerging pollutants as single chemicals or mixtures, i.e. triclosan, acridine, 17α-ethinylestradiol (EE2) and 3-nitrobenzanthrone (3-NBA). Assays evaluated effects on aquatic organisms from three different trophic levels (algae, daphnids, zebrafish embryos) and mechanism-specific effects using in vitro estrogenicity (ER-Luc, YES) and mutagenicity (Ames fluctuation) assays. The test battery presented complementary sensitivity and specificity to evaluate the different blinded water extract spikes. Aquatic organisms differed in terms of sensitivity to triclosan (algae > daphnids > fish) and acridine (fish > daphnids > algae) spikes, confirming the complementary role of the three taxa for water quality assessment. Estrogenicity and mutagenicity assays identified with high precision the respective mechanism-specific effects of spikes even when non-specific toxicity occurred in mixture. For estrogenicity, although differences were observed between assays and models, EE2 spike relative induction EC50 values were comparable to the literature, and E2/EE2 equivalency factors reliably reflected the sample content. In the Ames, strong revertant induction occurred following 3-NBA spike incubation with the TA98 strain, which was of lower magnitude after metabolic transformation and when compared to TA100. Differences in experimental protocols, model organisms, and data analysis can be sources of variation, indicating that respective harmonized standard procedures should be followed when implementing bioassays in water monitoring. Together with other ongoing activities for the validation of a basic bioassay battery, the present study is an important step towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 104, p. 473-484
Keywords [en]
Triclosan, Acridine, 17α-ethinylestradiol, 3-Nitrobenzanthrone, Organism-level toxicity, Mechanism-specific toxicity
National Category
Earth and Related Environmental Sciences
Research subject
Enviromental Science
Identifiers
URN: urn:nbn:se:oru:diva-52050DOI: 10.1016/j.watres.2016.08.018ISI: 000386401900049PubMedID: 27585427Scopus ID: 2-s2.0-84983806043OAI: oai:DiVA.org:oru-52050DiVA, id: diva2:967388
Note

Funding Agencies:

European Marie Curie Initial Training Network EDA-EMERGE 290100

European FP7 Collaborative Project SOLUTIONS 603437

NORMAN Association

EDA-EMERGE Marie Curie ITN ESR1

French Ministry of Ecology P181

ONEMA

Available from: 2016-09-08 Created: 2016-09-08 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Keiter, Steffen

Search in DiVA

By author/editor
Keiter, Steffen
By organisation
School of Science and Technology
Earth and Related Environmental Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 233 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf