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Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
1982 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 43, no 6, 1256-1261 p.Article in journal (Refereed) Published
Abstract [en]

Spectrophotometric determination of the hydrolysis of fluorescein diacetate (FDA) was shown to be a simple, sensitive, and rapid method for determining microbial activity in soil and litter. FDA hydrolysis was studied in soil and straw incubated for up to 3h. Hydrolysis was found to increase linearly with soil addition. FDA hydrolysis by pure cultures of Fusarium culmorum increased linearly with mycelium addition both in shake cultures and after inoculation into sterile soil. FDA hydrolys is by Pseudomonas denitrificans increased linearly with biomass addition. The FDA hydrolytic activities in soil samples from different layers of an agricultural soil were correlated with respiration. Acetone was found to be suitable for terminating the reaction.

Place, publisher, year, edition, pages
Washington: American Society for Microbiology , 1982. Vol. 43, no 6, 1256-1261 p.
National Category
Microbiology
Research subject
Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-52194ISI: A1982NS82700005PubMedID: 16346026Scopus ID: 2-s2.0-0019966926OAI: oai:DiVA.org:oru-52194DiVA: diva2:971013
Available from: 2016-09-15 Created: 2016-09-15 Last updated: 2017-10-17Bibliographically approved

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