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Fermentation of lignocellulosic hydrolysate by the alternative industrial ethanol yeast Dekkera bruxellensis
Department of Microbiology, Uppsala Biocenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Microbiology, Uppsala Biocenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Microbiology, Uppsala Biocenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Molecular Biology, Uppsala Biocenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.
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2011 (English)In: Letters in Applied Microbiology, ISSN 0266-8254, E-ISSN 1472-765X, Vol. 53, no 1, 73-78 p.Article in journal (Refereed) Published
Abstract [en]

Aim: Testing the ability of the alternative ethanol production yeast Dekkera bruxellensis to produce ethanol from lignocellulose hydrolysate and comparing it to Saccharomyces cerevisiae.

Methods and Results: Industrial isolates of D. bruxellensis and S. cerevisiae were cultivated in small-scale batch fermentations of enzymatically hydrolysed steam exploded aspen sawdust. Different dilutions of hydrolysate were tested. None of the yeasts grew in undiluted or 1 : 2 diluted hydrolysate [final glucose concentration always adjusted to 40 g l(-1) (0.22 mol l(-1))]. This was most likely due to the presence of inhibitors such as acetate or furfural. In 1 : 5 hydrolysate, S. cerevisiae grew, but not D. bruxellensis, and in 1 : 10 hydrolysate, both yeasts grew. An external vitamin source (e.g. yeast extract) was essential for growth of D. bruxellensis in this lignocellulosic hydrolysate and strongly stimulated S. cerevisiae growth and ethanol production. Ethanol yields of 0 42 +/- 0 01 g ethanol (g glucose)(-1) were observed for both yeasts in 1 : 10 hydrolysate. In small-scale continuous cultures with cell recirculation, with a gradual increase in the hydrolysate concentration, D. bruxellensis was able to grow in 1 : 5 hydrolysate. In bioreactor experiments with cell recirculation, hydrolysate contents were increased up to 1 : 2 hydrolysate, without significant losses in ethanol yields for both yeasts and only slight differences in viable cell counts, indicating an ability of both yeasts to adapt to toxic compounds in the hydrolysate.

Conclusions: Dekkera bruxellensis and S. cerevisiae have a similar potential to ferment lignocellulose hydrolysate to ethanol and to adapt to fermentation inhibitors in the hydrolysate.

Significance and Impact of the study: This is the first study investigating the potential of D. bruxellensis to ferment lignocellulosic hydrolysate. Its high competitiveness in industrial fermentations makes D. bruxellensis an interesting alternative for ethanol production from those substrates.

Place, publisher, year, edition, pages
Malden, USA: Wiley-Blackwell, 2011. Vol. 53, no 1, 73-78 p.
Keyword [en]
Aspen sawdust, Dekkera bruxellensis, Lignocellulosic hydrolysate, Saccharomyces cerevisiae
National Category
Biocatalysis and Enzyme Technology Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-52202DOI: 10.1111/j.1472-765X.2011.03067.xISI: 000292560700011PubMedID: 21535044Scopus ID: 2-s2.0-79958770656OAI: oai:DiVA.org:oru-52202DiVA: diva2:971238
Note

Funding Agency:

MicroDrivE 

Available from: 2016-09-15 Created: 2016-09-15 Last updated: 2017-10-17Bibliographically approved

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CiteExportLink to record
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