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Microbial changes during storage of moist crimped cereal barley grain under Swedish farm conditions
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Microbiology, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.ORCID iD: 0000-0002-2059-9044
2010 (English)In: Animal Feed Science and Technology, ISSN 0377-8401, E-ISSN 1873-2216, Vol. 156, no 1-2, 37-46 p.Article in journal (Refereed) Published
Abstract [en]

This Study investigated feed hygiene during airtight storage of non-dried barley grain under farm conditions. Microorganisms on the grain were sampled and quantified in seven Swedish firms throughout the storage period using culture dependent methods. The dominant lactic acid bacteria (LAB) and yeasts were identified by rRNA gene sequencing and moulds by morphological characterisation. Moisture content (MC) and pH of the grain were also monitored. It was difficult to obtain the optimal MC(0.30-0.45 g/g) that is necessary to initiate fermentation in the grain. Feed hygiene was maintained during storage of cereals when MC below 0.17 g/g. Intermediate MC (0.17-0.23 g/g) of the grains was conducive to mould growth, including growth of potential producers of mycotoxins, which can diminish feed safety and nutritional value. Enterobacteriaceae were found in all barleys, even at low MC, but their numbers were substantially decreased when the number of LAB was high. True fermentation of moist crimped cereal grains was only obtained on one farm with all initial barley MC of 0.3 g/g. Here, LAB reached high numbers during storage, whereas numbers of spoilage microorganisms that may reduce feed hygiene decreased considerably. However, the pH of the barley did not differ among farms. Storage stability in airtight stored barley may thus be the result of low oxygen tension, viz. airtight storage, and competition for nutrients by the microorganisms, rather than formation of lactic acid. At harvest, Enterococcus caccae dominated the LAB Population in five barleys and Lactobacillus fermentum at the remaining two. The dominant yeast species were Aureobasidium pullulans, Cryptococcus wieringae, Kazachstania aerobia and Rhodotorula glutinis. After storage, L fermentum was dominant among the LAB. The yeast species composition was highly diverse and differed among barleys. Among others, C. wieringae, Debaryomyces hansenii, K. aerobia, R. glutinis and Sporobolomyces ruberrimus were detected. This Study shows that the microbial population in airtight stored moist barley is highly diverse and not predictable. Thus, it may be necessary to influence the microbial population in the storage system by adding a starter culture.

Place, publisher, year, edition, pages
Amsterdam, Netherlands: Elsevier, 2010. Vol. 156, no 1-2, 37-46 p.
Keyword [en]
Cereal grains, Microbial population, Yeast, Lactic acid bacteria, Moulds, rRNA gene sequencing
National Category
Food Engineering
URN: urn:nbn:se:oru:diva-52276DOI: 10.1016/j.anifeedsci.2009.12.007ISI: 000275559900005ScopusID: 2-s2.0-75849139041OAI: oai:DiVA.org:oru-52276DiVA: diva2:971441

Funding Agencies:

Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS)  

Thematic Research Program MicroDrivE(Microbialy Derived Energy) 

Available from: 2016-09-16 Created: 2016-09-16 Last updated: 2016-09-16Bibliographically approved

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Schnürer, JohanPassoth, Volkmar
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