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Antifungal activity of chitinolytic bacteria isolated from airtight stored cereal grain
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
1998 (English)In: Canadian journal of microbiology (Print), ISSN 0008-4166, E-ISSN 1480-3275, Vol. 44, no 2, p. 121-127Article in journal (Refereed) Published
Abstract [en]

Chitinolytic bacteria are used as biocontrol agents of plant pathogenic fungi. They might also potentially inhibit growth of molds, e.g., Aspergillus spp. and Penicillium spp., in stored plant material. We isolated chitinolytic bacteria from airtight stored cereal grain and evaluated their antifungal capacity. Between 0.01 and 0.5% of the total aerobic counts were chitinolytic bacteria. Gram-negative bacteria, mainly Pseudomonadaceae, constituted approximately 80% of the chitinolytic population. Gram-positive isolates belonged predominantly to the Corynebacterium-Arthrobacter group, Streptomyces, and Bacillus. Chitinolytic activity was evaluated using culture filtrates from chitin-grown isolates as the release of p-nitrophenol from p-nitrophenyl N,N'-diacetylchitobiose and as the formation of clearing zones on chitin agar. No correlation between chitinolytic activity and antifungal effects was found when challenging Penicillium roqueforti Dierckx with bacterial isolates on chitin agar in a dual culture bioassay. Fungal hyphae frequently grew seemingly unaffected through the bacterial colony of a high chitinase producer on colloidal chitin. Only 4% of the chitinolytic isolates had strong effects on fungal growth. Among these, Streptomyces halstedii (K122) and Streptomyces coelicolor (K139) inhibited growth of a broad range of fungi. Streptomyces halstedii affected hyphal morphology and decreased the radial growth rate of all fungi investigated. These effects were not caused by volatile metabolites, polyenes, or N-carbamoyl-D-glucosamine.

Place, publisher, year, edition, pages
NRC Research Press, 1998. Vol. 44, no 2, p. 121-127
Keywords [en]
antifungal, chitinase, Streptomyces halstedii, Streptomyces coelicolor
National Category
Biochemistry and Molecular Biology Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-52289DOI: 10.1139/cjm-44-2-121ISI: 000072780000005Scopus ID: 2-s2.0-0031968483OAI: oai:DiVA.org:oru-52289DiVA, id: diva2:971507
Available from: 2016-09-16 Created: 2016-09-16 Last updated: 2017-11-21Bibliographically approved

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Schnürer, Johan

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