oru.sePublikationer
Change search
ReferencesLink to record
Permanent link

Direct link
Genetic characterization of the nonconventional yeast Hansenula anomala
State Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia; Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
State Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia; Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
2001 (English)In: Research in Microbiology, ISSN 0923-2508, E-ISSN 1769-7123, Vol. 152, no 6, 551-562 p.Article in journal (Refereed) Published
Abstract [en]

We describe genetic, molecular and taxonomic characteristics of the yeast Hansenula anomala. Pulsed-field gel electrophoresis of chromosomal DNAs from 19 H. anomala strains and related species indicated that H. anomala had a clearly different karyotype. Chromosome length polymorphism of the H. anomala strains was independent of their geographic origin and source of isolation. The strains were classified into four groups of similar karyotypes and one strain showed a unique profile. The sizes of chromosomes ranged from 850 to 3500 kb in different strains. The haploid chromosome number of H. anomala is at least nine. We have found RAPD primers discriminating at both the species and strain levels. All the primers tested except the M13 core sequence generated unique patterns with most strains. The results indicate the usefulness of PCR analysis with primer M13 for identification of the H. anomala species. Screening of the CBS (Utrecht) collection strains of H. anomala showed that they are rather difficult objects for genetic hybridization analysis. The strains have low fertility, viz. very poor sporulation, low mating type activities and, as a rule, nonviable ascospores. The majority of the hybrids obtained are polyploid, probably tetraploid, as judged by the segregation of control auxotrophic markers. Nevertheless, some monosporic cultures of the strains studied, including the biocontrol yeast J121, formed diploid hybrids with regular meiotic segregation of control auxotrophic markers. As a rule, H. anomala isolates are homothallic, showing delayed self-diploidization. Rare stable heterothallic strains of H. anomala also occur.

Place, publisher, year, edition, pages
Elsevier, 2001. Vol. 152, no 6, 551-562 p.
Keyword [en]
Hansenula anomala, Pichia anomala, taxogenetics, polyploidy, karyotyping, PCR analysis, D1-D2 sequence
National Category
Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-52306DOI: 10.1016/S0923-2508(01)01229-3ISI: 000170483500004PubMedID: 11501673ScopusID: 2-s2.0-0034947028OAI: oai:DiVA.org:oru-52306DiVA: diva2:971821
Available from: 2016-09-19 Created: 2016-09-16 Last updated: 2016-09-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Schnürer, Johan
In the same journal
Research in Microbiology
Microbiology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 35 hits
ReferencesLink to record
Permanent link

Direct link